AAC
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bartlett, K.
Right arrow Articles by Wasan, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bartlett, K.
Right arrow Articles by Wasan, K. M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, January 2004, p. 333-336, Vol. 48, No. 1
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.1.333-336.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of Heat-Treated Amphotericin B on Renal and Fungal Cytotoxicity

Karen Bartlett,1 Edwin Yau,2 Scott C. Hartsel,3 Alison Hamer,2 Gina Tsai,2 Dan Bizzotto,4 and Kishor M. Wasan2*

School of Occupational and Environmental Hygiene,1 Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3,2 Department of Chemistry, University of Wisconsin—Eau Claire, Eau Claire, Wisconsin,3 Department of Chemistry, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z14

Received 15 April 2003/ Returned for modification 19 July 2003/ Accepted 7 September 2003

The purpose of this investigation was to determine the cytotoxicity of amphotericin B (AMB; trade name Fungizone [FZ]) following the administration of FZ and a heat-treated form of FZ (HFZ) to LLC-PK1 pig kidney cells and Cryptococcus neoformans var. gattii cells. HFZ was significantly less toxic to kidney cells than FZ at all concentrations tested. For both FZ and HFZ, the concentration range which resulted in a 50% reduction of the growth of fungal cells was 0.125 to 1 mg/ml. These findings suggest that heat treatment decreases AMB's renal cytotoxicity without modifying its antifungal activity.

* Corresponding author. Mailing address: Faculty of Pharmaceutical Sciences, The University of British Columbia, 2146 East Mall, Vancouver, British Columbia, Canada V6T 1Z3. Phone: (604) 822-4889. Fax: (604) 822-3035. E-mail: kwasan{at}interchange.ubc.ca.

Antimicrobial Agents and Chemotherapy, January 2004, p. 333-336, Vol. 48, No. 1
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.1.333-336.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Clin. Vaccine Immunol. Clin. Microbiol. Rev.
J. Clin. Microbiol. ALL ASM JOURNALS

Copyright © 2004 by the American Society for Microbiology. All rights reserved.