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Antimicrobial Agents and Chemotherapy, January 2004, p. 358-359, Vol. 48, No. 1
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.1.358-359.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sequencing of Neisseria meningitidis penA Gene: the Key to Success in Defining Penicillin G Breakpoints

Luisa Arreaza, Celia Salcedo, Belén Alcalá, María José Uría, Raquel Abad, Rocío Enríquez, and Julio A. Vázquez*

Reference Laboratory for Meningococci, National Center of Microbiology, Institute of Health Carlos III, Madrid, Spain

Received 17 June 2003/ Returned for modification 18 August 2003/ Accepted 26 September 2003

Testing of susceptibility to penicillin G by E-test and sequencing of an internal fragment of the penA gene were done for 43 meningococcal strains. Those strains for which the MIC was >=0.094 µg/ml showed mosaic alleles, so 0.094 µg/ml is suggested as the penicillin G intermediate breakpoint when E-test is used.

* Corresponding author. Mailing address: Reference Laboratory for Meningococci, National Center of Microbiology, Institute of Health Carlos III, 28220 Majadahonda, Madrid, Spain. Phone: 34915097901. Fax: 34915097966. E-mail: jvazquez{at}isciii.es.

Antimicrobial Agents and Chemotherapy, January 2004, p. 358-359, Vol. 48, No. 1
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.1.358-359.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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