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Antimicrobial Agents and Chemotherapy, October 2004, p. 4012-4015, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.4012-4015.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Prevalence of Mutations within the Quinolone Resistance-Determining Region of gyrA, gyrB, parC, and parE and Association with Antibiotic Resistance in Quinolone-Resistant Salmonella enterica

Deborah J. Eaves,1 Luke Randall,2 Douglas T. Gray,1 Antony Buckley,1 Martin J. Woodward,2 Allan P. White,3 and Laura J. V. Piddock1*

Antimicrobial Agents Research Group, Division of Immunity and Infection,1 Statistical Advisory Service,3 Department of Food and Environmental Safety, Veterinary Laboratories Agency, New Haw, Surrey2

Received 21 January 2004/ Returned for modification 23 February 2004/ Accepted 1 June 2004

Salmonella enterica isolates (n = 182) were examined for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE. The frequency, location, and type of GyrA substitution varied with the serovar. Mutations were found in parC that encoded Thr57-Ser, Thr66-Ile, and Ser80-Arg substitutions. Mutations in the gyrB quinolone resistance-determining region were located at codon Tyr420-Cys or Arg437-Leu. Novel mutations were also found in parE encoding Glu453-Gly, His461-Tyr, Ala498-Thr, Val512-Gly, and Ser518-Cys. Although it is counterintuitive, isolates with a mutation in both gyrA and parC were more susceptible to ciprofloxacin than were isolates with a mutation in gyrA alone.

* Corresponding author. Mailing address: Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom. Phone: (44) 121-414-6966. Fax: (44) 121-414-3454. E-mail: l.j.v.piddock{at}bham.ac.uk.

Antimicrobial Agents and Chemotherapy, October 2004, p. 4012-4015, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.4012-4015.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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