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Antimicrobial Agents and Chemotherapy, October 2004, p. 4023-4026, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.4023-4026.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Peptide Nucleic Acid-Mediated Competitive PCR Clamping for Detection of Rifampin-Resistant Mycobacterium tuberculosis

Tomotada Iwamoto* and Toshiaki Sonobe

Department of Microbiology, Kobe Institute of Health, Chuo-ku, Kobe, Japan

Received 11 February 2004/ Returned for modification 28 April 2004/ Accepted 27 May 2004

Peptide nucleic acid-mediated competitive PCR clamping, which can selectively amplify mutant alleles, was developed to detect mutations in four codons (513, 516, 526, and 531) of the rpoB gene in Mycobacterium tuberculosis strains. This simple method successfully identified the mutations in all 40 of the M. tuberculosis strains tested.

* Corresponding author. Mailing address: Department of Microbiology, Kobe Institute of Health, 4-6 Minatojima-nakamachi, Chuo-ku, Kobe 650-0046, Japan. Phone: 81-78-302-6251. Fax: 81-78-302-0894. E-mail: kx2t-iwmt{at}asahi-net.or.jp.

Antimicrobial Agents and Chemotherapy, October 2004, p. 4023-4026, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.4023-4026.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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