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Antimicrobial Agents and Chemotherapy, October 2004, p. 4059-4062, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.4059-4062.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum ß-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay

Gerhard F. Weldhagen*

Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, and National Health Laboratory Service, Pretoria, South Africa

Received 23 March 2004/ Returned for modification 27 May 2004/ Accepted 19 June 2004

The LightCycler was compared to nested PCR for the detection of blaGES/IBC genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a blaGES/IBC gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.

* Mailing address: Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria, 0001, South Africa. Phone: 02712-319 2170. Fax: 02712-321 9456. E-mail: gweldhagen{at}medic.up.ac.za.

Antimicrobial Agents and Chemotherapy, October 2004, p. 4059-4062, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.4059-4062.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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