This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Girlich, D.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Girlich, D.
Right arrow Articles by Nordmann, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2004, p. 4217-4225, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4217-4225.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

OXA-60, a Chromosomal, Inducible, and Imipenem-Hydrolyzing Class D ß-Lactamase from Ralstonia pickettii

Delphine Girlich, Thierry Naas, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France

Received 30 April 2004/ Returned for modification 19 June 2004/ Accepted 28 July 2004

A chromosomally encoded oxacillinase, OXA-22, had been characterized from Ralstonia pickettii PIC-1 that did not explain by itself the resistance profile of this strain to ß-lactams. Thus, further analysis of the genetic background of this species led to the identification of another oxacillinase, OXA-60, that was expressed only after ß-lactam induction. This chromosomally encoded oxacillinase shared 19% amino acid identity with OXA-22. It has a narrow-spectrum hydrolysis profile that includes imipenem. OXA-60-like enzymes were identified in several R. pickettii strains. Gene inactivation and induction studies of the blaOXA-60 and blaOXA-22 genes in R. pickettii identified the relative contribution of each oxacillinase to the resistance profile of R. pickettii to ß-lactams.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cédex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, November 2004, p. 4217-4225, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4217-4225.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Doi, Y., Poirel, L., Paterson, D. L., Nordmann, P. (2008). Characterization of a Naturally Occurring Class D {beta}-Lactamase from Achromobacter xylosoxidans. Antimicrob. Agents Chemother. 52: 1952-1956 [Abstract] [Full Text]  
  • Queenan, A. M., Bush, K. (2007). Carbapenemases: the Versatile {beta}-Lactamases. Clin. Microbiol. Rev. 20: 440-458 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Nordmann, P. (2006). Regulation of class D beta-lactamase gene expression in Ralstonia pickettii.. Microbiology 152: 2661-2672 [Abstract] [Full Text]  
  • Schneider, I., Queenan, A. M., Bauernfeind, A. (2006). Novel Carbapenem-Hydrolyzing Oxacillinase OXA-62 from Pandoraea pnomenusa.. Antimicrob. Agents Chemother. 50: 1330-1335 [Abstract] [Full Text]  
  • Walther-Rasmussen, J., Hoiby, N. (2006). OXA-type carbapenemases. J Antimicrob Chemother 57: 373-383 [Abstract] [Full Text]  
  • Heritier, C., Poirel, L., Fournier, P.-E., Claverie, J.-M., Raoult, D., Nordmann, P. (2005). Characterization of the Naturally Occurring Oxacillinase of Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 4174-4179 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»