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Antimicrobial Agents and Chemotherapy, November 2004, p. 4217-4225, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4217-4225.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

OXA-60, a Chromosomal, Inducible, and Imipenem-Hydrolyzing Class D ß-Lactamase from Ralstonia pickettii

Delphine Girlich, Thierry Naas, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France

Received 30 April 2004/ Returned for modification 19 June 2004/ Accepted 28 July 2004

A chromosomally encoded oxacillinase, OXA-22, had been characterized from Ralstonia pickettii PIC-1 that did not explain by itself the resistance profile of this strain to ß-lactams. Thus, further analysis of the genetic background of this species led to the identification of another oxacillinase, OXA-60, that was expressed only after ß-lactam induction. This chromosomally encoded oxacillinase shared 19% amino acid identity with OXA-22. It has a narrow-spectrum hydrolysis profile that includes imipenem. OXA-60-like enzymes were identified in several R. pickettii strains. Gene inactivation and induction studies of the blaOXA-60 and blaOXA-22 genes in R. pickettii identified the relative contribution of each oxacillinase to the resistance profile of R. pickettii to ß-lactams.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cédex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, November 2004, p. 4217-4225, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4217-4225.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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