This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, A. S. G.
Right arrow Articles by Wong, S. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, A. S. G.
Right arrow Articles by Wong, S. Y.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2004, p. 4447-4449, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4447-4449.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel Mutations within the embB Gene in Ethambutol-Susceptible Clinical Isolates of Mycobacterium tuberculosis

Ann S. G. Lee,1* Siti Noor Khadijah Othman,1 Yu Min Ho,1 and Sin Yew Wong2

Division of Medical Sciences, National Cancer Centre,1 Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore2

Received 30 March 2004/ Returned for modification 10 May 2004/ Accepted 15 July 2004

Genetic analysis of the embB gene revealed mutations in 17 (68%) of 25 ethambutol (EMB) resistant isolates (M306I, M306V, M306L, Q497R) but also in 4 (20%) of 20 EMB-susceptible isolates of Mycobacterium tuberculosis, namely, an ATG->ATM substitution resulting in M306I, G406N, and the novel alterations M423I and A659T.

* Corresponding author. Mailing address: Division of Medical Sciences, National Cancer Centre, Singapore 169610, Republic of Singapore. Phone: 65 6436 8313. Fax: 65 6372 0161. E-mail: dmslsg{at}nccs.com.sg.

Antimicrobial Agents and Chemotherapy, November 2004, p. 4447-4449, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4447-4449.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Goude, R., Amin, A. G., Chatterjee, D., Parish, T. (2009). The Arabinosyltransferase EmbC Is Inhibited by Ethambutol in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 53: 4138-4146 [Abstract] [Full Text]  
  • Starks, A. M., Gumusboga, A., Plikaytis, B. B., Shinnick, T. M., Posey, J. E. (2009). Mutations at embB Codon 306 Are an Important Molecular Indicator of Ethambutol Resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 53: 1061-1066 [Abstract] [Full Text]  
  • Safi, H., Sayers, B., Hazbon, M. H., Alland, D. (2008). Transfer of embB Codon 306 Mutations into Clinical Mycobacterium tuberculosis Strains Alters Susceptibility to Ethambutol, Isoniazid, and Rifampin. Antimicrob. Agents Chemother. 52: 2027-2034 [Abstract] [Full Text]  
  • Shi, R., Zhang, J., Otomo, K., Zhang, G., Sugawara, I. (2007). Lack of Correlation between embB Mutation and Ethambutol MIC in Mycobacterium tuberculosis Clinical Isolates from China. Antimicrob. Agents Chemother. 51: 4515-4517 [Abstract] [Full Text]  
  • Shen, X., Shen, G.-m., Wu, J., Gui, X.-h., Li, X., Mei, J., DeRiemer, K., Gao, Q. (2007). Association between embB Codon 306 Mutations and Drug Resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 51: 2618-2620 [Abstract] [Full Text]  
  • Plinke, C., Rusch-Gerdes, S., Niemann, S. (2006). Significance of Mutations in embB Codon 306 for Prediction of Ethambutol Resistance in Clinical Mycobacterium tuberculosis Isolates.. Antimicrob. Agents Chemother. 50: 1900-1902 [Abstract] [Full Text]  
  • Dong, X., Bhamidi, S., Scherman, M., Xin, Y., McNeil, M. R. (2006). Development of a Quantitative Assay for Mycobacterial Endogenous Arabinase and Ensuing Studies of Arabinase Levels and Arabinan Metabolism in Mycobacterium smegmatis. Appl. Environ. Microbiol. 72: 2601-2605 [Abstract] [Full Text]  
  • Hazbon, M. H., Bobadilla del Valle, M., Guerrero, M. I., Varma-Basil, M., Filliol, I., Cavatore, M., Colangeli, R., Safi, H., Billman-Jacobe, H., Lavender, C., Fyfe, J., Garcia-Garcia, L., Davidow, A., Brimacombe, M., Leon, C. I., Porras, T., Bose, M., Chaves, F., Eisenach, K. D., Sifuentes-Osornio, J., Ponce de Leon, A., Cave, M. D., Alland, D. (2005). Role of embB Codon 306 Mutations in Mycobacterium tuberculosis Revisited: a Novel Association with Broad Drug Resistance and IS6110 Clustering Rather than Ethambutol Resistance. Antimicrob. Agents Chemother. 49: 3794-3802 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»