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Antimicrobial Agents and Chemotherapy, November 2004, p. 4485-4487, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4485-4487.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nonisotopic, Semiautomated Plasmodium falciparum Bioassay for Measurement of Antimalarial Drug Levels in Serum or Plasma

Harald Noedl,1,2* Paktiya Teja-Isavadharm,1 and Robert Scott Miller1

Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand,1 Department of Specific Prophylaxis and Tropical Medicine, Institute of Pathophysiology, University of Vienna, Vienna, Austria2

Received 8 January 2004/ Returned for modification 24 May 2004/ Accepted 22 July 2004

A simple, nonisotopic, semiautomated bioassay for the measurement of antimalarial drug levels in plasma or serum based on the quantitation of histidine-rich protein II in malaria culture is presented. The assay requires only small sample volumes and was found to be highly sensitive and reproducible. The results closely paralleled those obtained with isotopic bioassays (R = 0.988, P < 0.001) and high-performance liquid chromatography-electrochemical detection (R = 0.978, P < 0.001).

* Corresponding author. Mailing address: Department of Specific Prophylaxis and Tropical Medicine, Institute of Pathophysiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria. Phone: 43 01 4277 64882. Fax: 43 1 403 83 43 90. E-mail: harald.noedl{at}univie.ac.at.

Antimicrobial Agents and Chemotherapy, November 2004, p. 4485-4487, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4485-4487.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.