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Antimicrobial Agents and Chemotherapy, December 2004, p. 4528-4531, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4528-4531.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

TEM-121, a Novel Complex Mutant of TEM-Type ß-Lactamase from Enterobacter aerogenes

Laurent Poirel,{dagger} Hedi Mammeri,{dagger} and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France

Received 31 March 2004/ Returned for modification 31 May 2004/ Accepted 29 August 2004

Enterobacter aerogenes clinical isolate LOR was resistant to penicillins and ceftazidime but susceptible to cefuroxime, cephalothin, cefoxitin, cefotaxime, ceftriaxone, and cefepime. PCR and cloning experiments from this strain identified a novel TEM-type ß-lactamase (TEM-121) differing by five amino acid substitutions from ß-lactamase TEM-2 (Glu104Lys, Arg164Ser, Ala237Thr, Glu240Lys, and Arg244Ser) and by only one amino acid change from the extended-spectrum ß-lactamase (ESBL) TEM-24 (Arg244Ser), with the last substitution also being identified in the inhibitor-resistant ß-lactamase IRT-2. Kinetic parameters indicated that TEM-121 hydrolyzed ceftazidime and aztreonam (like TEM-24) and was inhibited weakly by clavulanic acid and strongly by tazobactam. Thus, TEM-121 is a novel complex mutant TEM ß-lactamase (CMT-4) combining the kinetic properties of an ESBL and an inhibitor-resistant TEM enzyme.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

{dagger} L.P. and H.M. contributed equally to this work.

Antimicrobial Agents and Chemotherapy, December 2004, p. 4528-4531, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4528-4531.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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