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Antimicrobial Agents and Chemotherapy, December 2004, p. 4886-4888, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4886-4888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli

Rodrigo Alonso, Estibaliz Mateo, Cecilia Girbau, Estibaliz Churruca, Irati Martinez, and Aurora Fernández-Astorga*

Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Vitoria-Gasteiz, Spain

Received 28 May 2004/ Returned for modification 20 July 2004/ Accepted 3 September 2004

A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.

* Corresponding author. Mailing address: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain. Phone: 34 945 013909. Fax: 34 945 013014. E-mail: oipfeasa{at}vc.ehu.es.

Antimicrobial Agents and Chemotherapy, December 2004, p. 4886-4888, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4886-4888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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