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Antimicrobial Agents and Chemotherapy, February 2004, p. 608-611, Vol. 48, No. 2
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.2.608-611.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Replacement of ParC {alpha}4 Helix with That of GyrA Increases the Stability and Cytotoxicity of Topoisomerase IV-Quinolone-DNA Ternary Complexes

Emily S. Pfeiffer and Hiroshi Hiasa*

Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Received 1 September 2003/ Returned for modification 13 October 2003/ Accepted 30 October 2003

Replacement of the {alpha}4 helix of ParC with that of GyrA increased the stability of topoisomerase IV-quinolone-DNA ternary complexes. This mutant topoisomerase IV-mediated cell killing was more efficient than topoisomerase IV-mediated cell killing in Escherichia coli. Thus, the {alpha}4 helix plays critical roles in determining the stability and the cytotoxicity of ternary complexes.

* Corresponding author. Mailing address: 6-120 Jackson Hall, 321 Church St. SE, Minneapolis, MN 55455. Phone: (612) 626-3101. Fax: (612) 625-8408. E-mail: hiasa001{at}tc.umn.edu.

Antimicrobial Agents and Chemotherapy, February 2004, p. 608-611, Vol. 48, No. 2
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.2.608-611.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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