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Antimicrobial Agents and Chemotherapy, February 2004, p. 612-614, Vol. 48, No. 2
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.2.612-614.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Environment and Transcription of ampC in an Acinetobacter baumannii Clinical Isolate

Heidi Segal,1 E. C. Nelson,1 and B. Gay Elisha1,2*

Department of Medical Microbiology, University of Cape Town,1 The National Health Laboratory Service, Cape Town, South Africa2

Received 31 July 2003/ Returned for modification 13 October 2003/ Accepted 3 November 2003

An ampC gene was cloned from a clinical isolate of Acinetobacter baumannii (strain RAN). DNA sequencing and primer extension studies showed that ampC is transcribed from a promoter contained within a putative insertion sequence element which has been found to abut several different genes in Acinetobacter spp.

* Corresponding author. Mailing address: Department of Medical Microbiology, Medical School, UCT, Anzio Road, Observatory 7925, Cape Town South Africa. Phone: (27) (21) 4066378. Fax: (27) (21) 4488153. E-mail: gelisha{at}curie.uct.ac.za.

Antimicrobial Agents and Chemotherapy, February 2004, p. 612-614, Vol. 48, No. 2
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.2.612-614.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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