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Antimicrobial Agents and Chemotherapy, March 2004, p. 1032-1033, Vol. 48, No. 3
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.3.1032-1033.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vitro Evolution Predicts that the IMP-1 Metallo-ß-Lactamase Does Not Have the Potential To Evolve Increased Activity against Imipenem

Biology Department, University of Rochester, Rochester, New York

Received 31 July 2003/ Returned for modification 20 October 2003/ Accepted 11 November 2003

In vitro evolution was used to predict whether the IMP-1 metallo-ß-lactamase has the potential to evolve an increased ability to confer resistance to imipenem. Screening of eight libraries containing 9.8 x 10⁶ ± 1.4 x 10⁶ (mean ± standard error) variants per library, with an average of 1.2 mutations per variant, detected no increased resistance to imipenem. The results predict, with >99.9% confidence, that even under intense selection the IMP-1 ß-lactamase will not evolve to confer increased resistance to imipenem.

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