This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, M.
Right arrow Articles by Hooper, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, M.
Right arrow Articles by Hooper, D. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2004, p. 1295-1299, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1295-1299.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Emerging Plasmid-Mediated Quinolone Resistance Associated with the qnr Gene in Klebsiella pneumoniae Clinical Isolates in the United States

Minggui Wang,1,2 Daniel F. Sahm,3 George A. Jacoby,4 and David C. Hooper1*

Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts 02114,1 Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China,2 Focus Technologies, Herndon, Virginia 20171,3 Infectious Disease Department, Lahey Clinic, Burlington, Massachusetts 018054

Received 10 October 2003/ Returned for modification 19 December 2003/ Accepted 30 December 2003

Although quinolone resistance commonly results from chromosomal mutation, recent studies indicate that such resistance can also be transferred on plasmids carrying the gene responsible, qnr. One hundred ten ciprofloxacin-resistant clinical isolates of Klebsiella pneumoniae and Escherichia coli from the United States were screened for the qnr gene by PCR and Southern hybridization of plasmid DNA. Conjugation experiments were done with azide-resistant E. coli J53 as the recipient and selection with azide and sulfonamide, a resistance frequently linked to qnr. EcoRI and BamHI digests of qnr-hybridizing plasmids were subjected to electrophoresis on agarose gels and probed with qnr by Southern hybridization. qnr was detected in 8 (11.1%) of 72 K. pneumoniae strains. These eight positive strains were from six states in the United States. qnr was not found in any of the 38 E. coli strains tested. Quinolone resistance was transferred from seven of the eight probe-positive strains. Transconjugants with qnr-hybridizing plasmids had 32-fold increases in ciprofloxacin MICs relative to E. coli J53. For all eight strains, the sequence of qnr was identical to that originally reported. By size and restriction digests, four plasmids were related to the first-reported plasmid, pMG252, and three were different. Five new qnr plasmids encoded FOX-5 ß-lactamase, as did pMG252, but two others produced SHV-7 extended-spectrum ß-lactamase. Transferable plasmid-mediated quinolone resistance associated with qnr is now widely distributed in quinolone-resistant clinical strains of K. pneumoniae in the United States. Plasmid-determined quinolone resistance contributes to the increasing quinolone resistance of K. pneumoniae isolates and to the linkage previously observed between resistance to quinolones and the latest ß-lactam antibiotics.

* Corresponding author. Mailing address: Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit St., Boston, MA 02114-2696. Phone: (617) 726-3812. Fax: (617) 726-7416. E-mail: dhooper{at}partners.org.

Antimicrobial Agents and Chemotherapy, April 2004, p. 1295-1299, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1295-1299.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Strahilevitz, J., Jacoby, G. A., Hooper, D. C., Robicsek, A. (2009). Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat. Clin. Microbiol. Rev. 22: 664-689 [Abstract] [Full Text]  
  • Cano, M. E., Rodriguez-Martinez, J. M., Aguero, J., Pascual, A., Calvo, J., Garcia-Lobo, J. M., Velasco, C., Francia, M. V., Martinez-Martinez, L. (2009). Detection of Plasmid-Mediated Quinolone Resistance Genes in Clinical Isolates of Enterobacter spp. in Spain. J. Clin. Microbiol. 47: 2033-2039 [Abstract] [Full Text]  
  • Sjolund-Karlsson, M., Folster, J. P., Pecic, G., Joyce, K., Medalla, F., Rickert, R., Whichard, J. M. (2009). Emergence of Plasmid-Mediated Quinolone Resistance among Non-Typhi Salmonella enterica Isolates from Humans in the United States. Antimicrob. Agents Chemother. 53: 2142-2144 [Abstract] [Full Text]  
  • Tamang, M. D., Seol, S. Y., Oh, J.-Y., Kang, H. Y., Lee, J. C., Lee, Y. C., Cho, D. T., Kim, J. (2008). Plasmid-Mediated Quinolone Resistance Determinants qnrA, qnrB, and qnrS among Clinical Isolates of Enterobacteriaceae in a Korean Hospital. Antimicrob. Agents Chemother. 52: 4159-4162 [Abstract] [Full Text]  
  • Sanchez-Cespedes, J., Blasco, M. D., Marti, S., Alba, V., Alcalde, E., Esteve, C., Vila, J. (2008). Plasmid-Mediated QnrS2 Determinant from a Clinical Aeromonas veronii Isolate. Antimicrob. Agents Chemother. 52: 2990-2991 [Full Text]  
  • Lascols, C., Podglajen, I., Verdet, C., Gautier, V., Gutmann, L., Soussy, C.-J., Collatz, E., Cambau, E. (2008). A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae. J. Bacteriol. 190: 5217-5223 [Abstract] [Full Text]  
  • Wu, J.-J., Ko, W.-C., Wu, H.-M., Yan, J.-J. (2008). Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005. J Antimicrob Chemother 61: 1234-1239 [Abstract] [Full Text]  
  • Cesaro, A., Bettoni, R. R. D., Lascols, C., Merens, A., Soussy, C. J., Cambau, E. (2008). Low selection of topoisomerase mutants from strains of Escherichia coli harbouring plasmid-borne qnr genes. J Antimicrob Chemother 61: 1007-1015 [Abstract] [Full Text]  
  • Xu, X., Wu, S., Ye, X., Liu, Y., Shi, W., Zhang, Y., Wang, M. (2007). Prevalence and Expression of the Plasmid-Mediated Quinolone Resistance Determinant qnrA1. Antimicrob. Agents Chemother. 51: 4105-4110 [Abstract] [Full Text]  
  • Wu, J.-J., Ko, W.-C., Tsai, S.-H., Yan, J.-J. (2007). Prevalence of Plasmid-Mediated Quinolone Resistance Determinants QnrA, QnrB, and QnrS among Clinical Isolates of Enterobacter cloacae in a Taiwanese Hospital. Antimicrob. Agents Chemother. 51: 1223-1227 [Abstract] [Full Text]  
  • Poirel, L., Leviandier, C., Nordmann, P. (2006). Prevalence and Genetic Analysis of Plasmid-Mediated Quinolone Resistance Determinants QnrA and QnrS in Enterobacteriaceae Isolates from a French University Hospital. Antimicrob. Agents Chemother. 50: 3992-3997 [Abstract] [Full Text]  
  • Lavigne, J.-P., Marchandin, H., Delmas, J., Bouziges, N., Lecaillon, E., Cavalie, L., Jean-Pierre, H., Bonnet, R., Sotto, A. (2006). qnrA in CTX-M-Producing Escherichia coli Isolates from France. Antimicrob. Agents Chemother. 50: 4224-4228 [Abstract] [Full Text]  
  • Soge, O. O., Adeniyi, B. A., Roberts, M. C. (2006). New antibiotic resistance genes associated with CTX-M plasmids from uropathogenic Nigerian Klebsiella pneumoniae. J Antimicrob Chemother 58: 1048-1053 [Abstract] [Full Text]  
  • Chen, Y.-T., Shu, H.-Y., Li, L.-H., Liao, T.-L., Wu, K.-M., Shiau, Y.-R., Yan, J.-J., Su, I.-J., Tsai, S.-F., Lauderdale, T.-L. (2006). Complete Nucleotide Sequence of pK245, a 98-Kilobase Plasmid Conferring Quinolone Resistance and Extended-Spectrum-{beta}-Lactamase Activity in a Clinical Klebsiella pneumoniae Isolate. Antimicrob. Agents Chemother. 50: 3861-3866 [Abstract] [Full Text]  
  • Park, C. H., Robicsek, A., Jacoby, G. A., Sahm, D., Hooper, D. C. (2006). Prevalence in the United States of aac(6')-Ib-cr Encoding a Ciprofloxacin-Modifying Enzyme. Antimicrob. Agents Chemother. 50: 3953-3955 [Abstract] [Full Text]  
  • Bonemann, G., Stiens, M., Puhler, A., Schluter, A. (2006). Mobilizable IncQ-Related Plasmid Carrying a New Quinolone Resistance Gene, qnrS2, Isolated from the Bacterial Community of a Wastewater Treatment Plant.. Antimicrob. Agents Chemother. 50: 3075-3080 [Abstract] [Full Text]  
  • Leavis, H. L., Willems, R. J. L., Top, J., Bonten, M. J. M. (2006). High-Level Ciprofloxacin Resistance from Point Mutations in gyrA and parC Confined to Global Hospital-Adapted Clonal Lineage CC17 of Enterococcus faecium.. J. Clin. Microbiol. 44: 1059-1064 [Abstract] [Full Text]  
  • Szabo, D., Melan, M. A., Hujer, A. M., Bonomo, R. A., Hujer, K. M., Bethel, C. R., Kristof, K., Paterson, D. L. (2005). Molecular Analysis of the Simultaneous Production of Two SHV-Type Extended-Spectrum Beta-Lactamases in a Clinical Isolate of Enterobacter cloacae by Using Single-Nucleotide Polymorphism Genotyping. Antimicrob. Agents Chemother. 49: 4716-4720 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Nordmann, P., Poirel, L. (2005). Emergence of plasmid-mediated resistance to quinolones in Enterobacteriaceae. J Antimicrob Chemother 56: 463-469 [Abstract] [Full Text]  
  • Robicsek, A., Sahm, D. F., Strahilevitz, J., Jacoby, G. A., Hooper, D. C. (2005). Broader Distribution of Plasmid-Mediated Quinolone Resistance in the United States. Antimicrob. Agents Chemother. 49: 3001-3003 [Abstract] [Full Text]  
  • Poirel, L., Van De Loo, M., Mammeri, H., Nordmann, P. (2005). Association of Plasmid-Mediated Quinolone Resistance with Extended-Spectrum {beta}-Lactamase VEB-1. Antimicrob. Agents Chemother. 49: 3091-3094 [Abstract] [Full Text]  
  • Pitout, J. D. D., Nordmann, P., Laupland, K. B., Poirel, L. (2005). Emergence of Enterobacteriaceae producing extended-spectrum {beta}-lactamases (ESBLs) in the community. J Antimicrob Chemother 56: 52-59 [Abstract] [Full Text]  
  • Jeong, J.-Y., Yoon, H. J., Kim, E. S., Lee, Y., Choi, S.-H., Kim, N. J., Woo, J. H., Kim, Y. S. (2005). Detection of qnr in Clinical Isolates of Escherichia coli from Korea. Antimicrob. Agents Chemother. 49: 2522-2524 [Abstract] [Full Text]  
  • Machado, E., Canton, R., Baquero, F., Galan, J.-C., Rollan, A., Peixe, L., Coque, T. M. (2005). Integron Content of Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Strains over 12 Years in a Single Hospital in Madrid, Spain. Antimicrob. Agents Chemother. 49: 1823-1829 [Abstract] [Full Text]  
  • Schwaber, M. J., Navon-Venezia, S., Schwartz, D., Carmeli, Y. (2005). High Levels of Antimicrobial Coresistance among Extended-Spectrum-{beta}-Lactamase-Producing Enterobacteriaceae. Antimicrob. Agents Chemother. 49: 2137-2139 [Abstract] [Full Text]  
  • Saga, T., Kaku, M., Onodera, Y., Yamachika, S., Sato, K., Takase, H. (2005). Vibrio parahaemolyticus Chromosomal qnr Homologue VPA0095: Demonstration by Transformation with a Mutated Gene of Its Potential To Reduce Quinolone Susceptibility in Escherichia coli. Antimicrob. Agents Chemother. 49: 2144-2145 [Full Text]  
  • Nazic, H., Poirel, L., Nordmann, P. (2005). Further Identification of Plasmid-Mediated Quinolone Resistance Determinant in Enterobacteriaceae in Turkey. Antimicrob. Agents Chemother. 49: 2146-2147 [Full Text]  
  • Jonas, D., Biehler, K., Hartung, D., Spitzmuller, B., Daschner, F. D. (2005). Plasmid-Mediated Quinolone Resistance in Isolates Obtained in German Intensive Care Units. Antimicrob. Agents Chemother. 49: 773-775 [Abstract] [Full Text]  
  • Hata, M., Suzuki, M., Matsumoto, M., Takahashi, M., Sato, K., Ibe, S., Sakae, K. (2005). Cloning of a Novel Gene for Quinolone Resistance from a Transferable Plasmid in Shigella flexneri 2b. Antimicrob. Agents Chemother. 49: 801-803 [Abstract] [Full Text]  
  • Mammeri, H., Van De Loo, M., Poirel, L., Martinez-Martinez, L., Nordmann, P. (2005). Emergence of Plasmid-Mediated Quinolone Resistance in Escherichia coli in Europe. Antimicrob. Agents Chemother. 49: 71-76 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»