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Antimicrobial Agents and Chemotherapy, April 2004, p. 1365-1368, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1365-1368.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of Amoxicillin-Clavulanic Acid on Human Fecal Flora in a Gnotobiotic Mouse Model Assessed with Fluorescence Hybridization Using Group-Specific 16S rRNA Probes in Combination with Flow Cytometry

Marie Claude Barc,1 François Bourlioux,1 Lionel Rigottier-Gois,2 Céline Charrin-Sarnel,1 Claire Janoir,1 Hélène Boureau,1 Joël Doré,2 and Anne Collignon1*

Unité associée INRA "Ecosystème Microbien Digestif et Santé," Faculté de Pharmacie, Université Paris-Sud, 92296 ChÂtenay-Malabry Cedex,1 Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78852 Jouy en Josas Cedex, France2

Received 25 July 2003/ Returned for modification 8 September 2003/ Accepted 24 December 2003

Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.

* Corresponding author. Mailing address: Université Paris-Sud, Faculté de Pharmacie, Département de Microbiologie, 92296 ChÂtenay-Malabry Cedex, France. Phone: (33) 1 46 83 55 49. Fax: (33) 1 46 83 55 37. E-mail: anne.collignon{at}cep.u-psud.fr.

Antimicrobial Agents and Chemotherapy, April 2004, p. 1365-1368, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1365-1368.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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