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Antimicrobial Agents and Chemotherapy, May 2004, p. 1807-1810, Vol. 48, No. 5
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.5.1807-1810.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy

Tyler N. Bennett, Michelle Paguio, Bojana Gligorijevic, Clement Seudieu, Andrew D. Kosar, Eugene Davidson, and Paul D. Roepe*

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Lombardi Cancer Center, Georgetown University, Washington DC 20057

Received 2 September 2003/ Returned for modification 27 October 2003/ Accepted 12 January 2004

We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [3H]hypoxanthine and [3H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.

* Corresponding author. Mailing address: Department of Chemistry, Georgetown University, 37th and O St. NW, Washington, DC 20057. Phone: (202) 687-7300. Fax: (202) 687-6209. E-mail roepep{at}georgetown.edu.

Antimicrobial Agents and Chemotherapy, May 2004, p. 1807-1810, Vol. 48, No. 5
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.5.1807-1810.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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