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Antimicrobial Agents and Chemotherapy, May 2004, p. 1879-1881, Vol. 48, No. 5
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.5.1879-1881.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Colorimetric Assay for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa

Michael M. Tunney,1* Gordon Ramage,2,{dagger} Tyler R. Field,2 Thomas F. Moriarty,1 and Douglas G. Storey2

The Clinical and Practice Research Group, School of Pharmacy, The Queen's University of Belfast, Belfast BT9 7BL, United Kingdom,1 Department of Biological Sciences, University of Calgary, Calgary, Canada T2N 1N42

Received 23 September 2003/ Returned for modification 17 December 2003/ Accepted 18 January 2004

A colorimetric assay based on the reduction of a tetrazolium salt {2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT)} for rapidly determining the susceptibility of Pseudomonas aeruginosa isolates to bactericidal antibiotics is described. There was excellent agreement between the tobramycin and ofloxacin MICs determined after 5 h using the XTT assay and after 18 h using conventional methods. The data suggests that an XTT-based assay could provide a useful method for rapidly determining the susceptibility of P. aeruginosa to bactericidal antibiotics.

* Corresponding author. Mailing address: The Clinical and Practice Research Group, School of Pharmacy, The Queen's University of Belfast, 97 Lisburn Rd., Belfast BT9 7BL, United Kingdom. Phone: 02890 272087. Fax: 02890 247794. E-mail: m.tunney{at}qub.ac.uk.

{dagger} Present address: Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, United Kingdom.

Antimicrobial Agents and Chemotherapy, May 2004, p. 1879-1881, Vol. 48, No. 5
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.5.1879-1881.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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