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Antimicrobial Agents and Chemotherapy, June 2004, p. 2153-2158, Vol. 48, No. 6
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.6.2153-2158.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Omp35, a New Enterobacter aerogenes Porin Involved in Selective Susceptibility to Cephalosporins

Charléric Bornet,1 Nathalie Saint,2 Lilia Fetnaci,1 Myrielle Dupont,1 Anne Davin-Régli,1 Claude Bollet,1 and Jean-Marie Pagès1*

Enveloppe Bactérienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculté de Médecine, Université de la Méditerranée, Marseille,1 Centre de Biochimie Structurale, UMR 5048 CNRS, 554 INSERM, Montpellier, France2

Received 4 December 2003/ Returned for modification 21 January 2004/ Accepted 5 February 2004

In Enterobacter aerogenes, ß-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. In ATCC 15038 strain, we observed a different pattern of porin production associated with a variable antibiotic susceptibility. We purified Omp35, which is expressed under conditions of low osmolality and analyzed its pore-forming properties in artificial membranes. This porin was found to be an OmpF-like protein with high conductance values. It showed a noticeably higher conductance compared to Omp36 and a specific location of WNYT residues in the L3 loop. The importance of the constriction region in the porin function suggests that this organization is involved in the level of susceptibility to negative large cephalosporins such as ceftriaxone by bacteria producing the Omp35 porin subfamily.

* Corresponding author. Mailing address: EA2197, Faculté de Médecine, 27 Bd Jean Moulin 13385 Marseille Cedex 05, France. Phone: (33) 4 91 32 45 87. Fax: (33) 4 91 32 46 06. E-mail: Jean-Marie.Pages{at}medecine.univ-mrs.fr.

Antimicrobial Agents and Chemotherapy, June 2004, p. 2153-2158, Vol. 48, No. 6
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.6.2153-2158.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kaczmarek, F. M., Dib-Hajj, F., Shang, W., Gootz, T. D. (2006). High-Level Carbapenem Resistance in a Klebsiella pneumoniae Clinical Isolate Is Due to the Combination of blaACT-1 {beta}-Lactamase Production, Porin OmpK35/36 Insertional Inactivation, and Down-Regulation of the Phosphate Transport Porin PhoE.. Antimicrob. Agents Chemother. 50: 3396-3406 [Abstract] [Full Text]  


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