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Antimicrobial Agents and Chemotherapy, June 2004, p. 2274-2276, Vol. 48, No. 6
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.6.2274-2276.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Regulatory Regions of smeDEF in Stenotrophomonas maltophilia Strains Expressing Different Amounts of the Multidrug Efflux Pump SmeDEF

Patricia Sanchez, Ana Alonso, and Jose L. Martinez*

Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain

Received 22 July 2003/ Returned for modification 3 November 2003/ Accepted 21 February 2004

The smeT-smeDEF region and the smeT gene, which encodes the smeDEF repressor, are highly polymorphic. Few changes in smeT might be associated with smeDEF overexpression. The results obtained with cellular extracts suggest that mutant SmeT proteins cannot bind to the operator and that other transcription factors besides SmeT are involved in the regulation of smeDEF expression.

* Corresponding author. Mailing address: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain. Phone: 34-91-5854542. Fax: 34-91-5854506. E-mail: jlmtnez{at}cnb.uam.es.

Antimicrobial Agents and Chemotherapy, June 2004, p. 2274-2276, Vol. 48, No. 6
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.6.2274-2276.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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