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Antimicrobial Agents and Chemotherapy, June 2004, p. 2308-2313, Vol. 48, No. 6
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.6.2308-2313.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

High-Level Resistance to Ceftazidime Conferred by a Novel Enzyme, CTX-M-32, Derived from CTX-M-1 through a Single Asp240-Gly Substitution

Monica Cartelle, Maria del Mar Tomas, Francisca Molina, Rita Moure, Rosa Villanueva, and German Bou*

Servicio de Microbiologia, Complejo Hospitalario Universitario Juan Canalejo, 15006 La Coruña, Spain

Received 27 June 2003/ Returned for modification 8 September 2003/ Accepted 13 February 2004

A clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (blaCTX-M-32) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed mutagenesis we demonstrated that this replacement is a key event in ceftazidime hydrolysis

* Corresponding author. Mailing address: Servicio de Microbiologia, Complejo Hospitalario Universitario Juan Canalejo, C/Xubias de Arriba s/n, 15006 La Coruña, Spain. Phone: 981-178000, ext. 21144. Fax: 981-178216. E-mail: germanbou{at}canalejo.org.

Antimicrobial Agents and Chemotherapy, June 2004, p. 2308-2313, Vol. 48, No. 6
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.6.2308-2313.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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