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Antimicrobial Agents and Chemotherapy, July 2004, p. 2742-2746, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2742-2746.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay

Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan,¹ Department of Medical Microbiology & Infectious Diseases, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands²

Received 10 November 2003/ Returned for modification 5 December 2003/ Accepted 27 February 2004

Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC₅₀]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC₅₀ of 0.5 to 1.0 mg/liter).

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