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Antimicrobial Agents and Chemotherapy, August 2004, p. 2845-2852, Vol. 48, No. 8
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.8.2845-2852.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

DNA Sequence Analysis of Regions Surrounding blaCMY-2 from Multiple Salmonella Plasmid Backbones

W. P. Giles,1 A. K. Benson,2 M. E. Olson,3 R. W. Hutkins,2 J. M. Whichard,4 P. L. Winokur,5 and P. D. Fey3,6*

Departments of Biology,1 Food Science, University of Nebraska—Lincoln, Lincoln,2 Departments of Internal Medicine,3 Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska,6 Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,4 Department of Internal Medicine, University of Iowa and Veterans Affairs Medical Center, Iowa City, Iowa5

Received 17 November 2003/ Returned for modification 7 February 2004/ Accepted 12 April 2004

The emergence in the United States of resistance to expanded-spectrum cephalosporin (e.g., ceftriaxone) within the salmonellae has been associated primarily with three large (>100-kb) plasmids (designated types A, B, and C) and one 10.1-kb plasmid (type D) that carry the blaCMY-2 gene. In the present study, the distribution of these four known blaCMY-2-carrying plasmids among 35 ceftriaxone-resistant Salmonella isolates obtained from 1998 to 2001 was examined. Twenty-three of these isolates were Salmonella enterica serotype Newport, 10 were Salmonella enterica serotype Typhimurium, 1 was Salmonella enterica serotype Agona, and 1 was Salmonella enterica serotype Reading. All 23 serotype Newport isolates carried a type C plasmid, and 5, 4, and 1 serovar Typhimurium isolate carried type B, A, and C plasmids, respectively. Both the serotype Agona and serotype Reading isolates carried type A plasmids. None of the isolates carried a type D plasmid. Hybridization data suggested that plasmid types A and C were highly related replicons. DNA sequencing revealed that the region surrounding blaCMY-2 was highly conserved in all three plasmid types analyzed (types B, C, and D) and was related to a region surrounding blaCMY-5 from the Klebsiella oxytoca plasmid pTKH11. These findings are consistent with a model in which blaCMY-2 has been disseminated primarily through plasmid transfer, and not by mobilization of the gene itself, to multiple Salmonella chromosomal backbones.

* Corresponding author. Mailing address: University of Nebraska Medical Center, 985400 Nebraska Medical Center, Omaha, NE 68198-5400. Phone: (402) 559-2122. Fax: (402) 559-5581. E-mail: pfey{at}unmc.edu.

Antimicrobial Agents and Chemotherapy, August 2004, p. 2845-2852, Vol. 48, No. 8
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.8.2845-2852.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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