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Genome-Wide Transcriptional Profiling of the Escherichia coli Response to a Proline-Rich Antimicrobial Peptide

Linda Tomasinsig,^1,2, Marco Scocchi,^2,, Romina Mettulio,² and Margherita Zanetti^1,2*

Department of Biomedical Sciences and Technology, University of Udine, Udine,¹ National Laboratory CIB, Area Science Park, Trieste, Italy²

Received 10 February 2004/ Returned for modification 8 March 2004/ Accepted 3 May 2004

Most antimicrobial peptides (AMPs) impair the viability of target bacteria by permeabilizing bacterial membranes. However, the proline-rich AMPs have been shown to kill susceptible organisms without causing significant membrane perturbation and may act by inhibiting the activity of bacterial targets. To gain initial insight into the events that follow interaction of a proline-rich peptide with bacterial cells, we used DNA macroarray technology to monitor transcriptional alterations of Escherichia coli in response to challenge with a subinhibitory concentration of the proline-rich Bac7(1-35). Substantial changes in the expression levels of 70 bacterial genes from various functional categories were detected. Among these, 26 genes showed decreased expression, while 44 genes, including genes that are potentially involved in bacterial resistance to antimicrobials, showed increased expression. The generation of a transcriptional response under the experimental conditions used is consistent with the ability of Bac7(1-35) to interact with bacterial components and affect biological processes in this organism.

L.T. and M.S. contributed equally to this work.

Present address: Department of Biochemistry, Biophysics, and Macromolecular Chemistry, University of Trieste, I-34127 Trieste, Italy.

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