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Antimicrobial Agents and Chemotherapy, September 2004, p. 3402-3406, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3402-3406.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sequence-Selective Recognition of Extended-Spectrum ß-Lactamase GES-2 by a Competitive, Peptide Nucleic Acid-Based Multiplex PCR Assay

Gerhard F. Weldhagen*

Department of Medical Microbiology, Pathology, Faculty of Health Sciences, University of Pretoria, and National Health Laboratory Service, Pretoria, South Africa

Received 12 January 2004/ Returned for modification 4 April 2004/ Accepted 2 May 2004

Extended-spectrum ß-lactamases (ESBLs) in Pseudomonas aeruginosa, such as GES-2, which compromises the efficacy of imipenem, tend to be geographically restricted. The CC-to-AA base pair substitution at positions 493 and 494 of the blaGES-2-coding region distinguishes this ESBL from blaGES-1 and the blaIBC-type genes, making it an ideal target for the development of a novel sequence-specific, peptide nucleic acid (PNA)-based multiplex PCR detection method. By using two primer pairs in conjunction with a PNA probe, this method provided an accurate means of identification of blaGES-2 compared to standard PCR and gene sequencing techniques when it was used to test 100 P. aeruginosa clinical isolates as well as previously published, well-described control strains encompassing all presently known genes in the blaGES-IBC ESBL family. This novel method has the potential to be used in large-scale, cost-effective screening programs for specific or geographically restricted ESBLs.

* Mailing address: Department of Medical Microbiology, Pathology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria, 0001, South Africa. Phone: 02712-319 2170. Fax: 02712-321 9456. E-mail: gweldhagen{at}medic.up.ac.za.

Antimicrobial Agents and Chemotherapy, September 2004, p. 3402-3406, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3402-3406.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poirel, L., Naas, T., Nordmann, P. (2006). Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum {beta}-Lactamases.. J. Clin. Microbiol. 44: 3008-3011 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Weldhagen, G. F. (2004). Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum {beta}-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay. Antimicrob. Agents Chemother. 48: 4059-4062 [Abstract] [Full Text]  


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