This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gibreel, A.
Right arrow Articles by Taylor, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gibreel, A.
Right arrow Articles by Taylor, D. E.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, September 2004, p. 3442-3450, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3442-3450.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet(O)-Mediated Tetracycline Resistance

Amera Gibreel,1,{dagger} Dobryan M. Tracz,1,{dagger} Lisa Nonaka,1,{ddagger} Trinh M. Ngo,1 Sean R. Connell,1,§ and Diane E. Taylor1,2*

Departments of Medical Microbiology and Immunology,1 Biological Sciences, University of Alberta, Edmonton, Alberta, Canada2

Received 26 February 2004/ Returned for modification 10 April 2004/ Accepted 19 May 2004

Of 203 human clinical isolates of Campylobacter jejuni from Alberta, Canada (1999 to 2002), 101 isolates (50%) were resistant to at least 64 µg of tetracycline/ml, with four isolates exhibiting higher levels of tetracycline resistance (512 µg/ml). In total, the MICs for 37% of tetracycline-resistant isolates (256 to 512 µg/ml) were higher than those previously reported in C. jejuni (64 to 128 µg/ml). In the tetracycline-resistant clinical isolates, 67% contained plasmids and all contained the tet(O) gene. Four isolates resistant to high levels of tetracycline (MIC = 512 µg/ml) contained plasmids carrying the tet(O) gene, which could be transferred to other isolates of C. jejuni. The tetracycline MICs for transconjugants were comparable to those of the donors. Cloning of tet(O) from the four high-level tetracycline-resistant isolates conferred an MIC of 32 µg/ml for Escherichia coli DH5{alpha}. In contrast, transfer to a strain of C. jejuni by using mobilization conferred an MIC of 128 µg/ml. DNA sequence analysis determined that the tet(O) genes encoding lower MICs (64 to 128 µg/ml) were identical to one other, although the tet(O) genes encoding a 512-µg/ml MIC demonstrated several nucleotide substitutions. The quinolone resistance determining region of four ciprofloxacin-resistant isolates (2%) was analyzed, and resistance was associated with a chromosomal mutation in the gyrA gene resulting in a Thr-86-Ile substitution. In addition, six kanamycin-resistant isolates contained large plasmids that carry the aphA-3 marker coding for 3'-aminoglycoside phosphotransferase. Resistance to erythromycin was not detected in 203 isolates. In general, resistance to most antibiotics in C. jejuni remains low, except for resistance to tetracycline, which has increased from about 8 to 50% over the past 20 years.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, 1-28 Medical Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2H7 Canada. Phone: (780) 492-4777. Fax: (780) 492-7521. E-mail: diane.taylor{at}ualberta.ca.

{dagger} A.G. and D.M.T. contributed equally to this work.

{ddagger} Present address: Center for Marine Environmental Studies (CMES) Ehime University, Matsuyama, 790-8577 Japan.

§ Present address: Institut für Medizinishe Physik und Biophysik, Universitätsklinikum Charite, 10117 Berlin, Germany.

Antimicrobial Agents and Chemotherapy, September 2004, p. 3442-3450, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3442-3450.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Habib, I., Miller, W. G., Uyttendaele, M., Houf, K., De Zutter, L. (2009). Clonal Population Structure and Antimicrobial Resistance of Campylobacter jejuni in Chicken Meat from Belgium. Appl. Environ. Microbiol. 75: 4264-4272 [Abstract] [Full Text]  
  • Gu, W., Siletzky, R. M., Wright, S., Islam, M., Kathariou, S. (2009). Antimicrobial Susceptibility Profiles and Strain Type Diversity of Campylobacter jejuni Isolates from Turkeys in Eastern North Carolina. Appl. Environ. Microbiol. 75: 474-482 [Abstract] [Full Text]  
  • Poly, F., Read, T. D., Chen, Y.-H., Monteiro, M. A., Serichantalergs, O., Pootong, P., Bodhidatta, L., Mason, C. J., Rockabrand, D., Baqar, S., Porter, C. K., Tribble, D., Darsley, M., Guerry, P. (2008). Characterization of Two Campylobacter jejuni Strains for Use in Volunteer Experimental-Infection Studies. Infect. Immun. 76: 5655-5667 [Abstract] [Full Text]  
  • Sahin, O., Plummer, P. J., Jordan, D. M., Sulaj, K., Pereira, S., Robbe-Austerman, S., Wang, L., Yaeger, M. J., Hoffman, L. J., Zhang, Q. (2008). Emergence of a Tetracycline-Resistant Campylobacter jejuni Clone Associated with Outbreaks of Ovine Abortion in the United States. J. Clin. Microbiol. 46: 1663-1671 [Abstract] [Full Text]  
  • Gibreel, A., Wetsch, N. M., Taylor, D. E. (2007). Contribution of the CmeABC Efflux Pump to Macrolide and Tetracycline Resistance in Campylobacter jejuni. Antimicrob. Agents Chemother. 51: 3212-3216 [Abstract] [Full Text]  
  • Quinn, T., Bolla, J.-M., Pages, J.-M., Fanning, S. (2007). Antibiotic-resistant Campylobacter: could efflux pump inhibitors control infection?. J Antimicrob Chemother 59: 1230-1236 [Abstract] [Full Text]  
  • Dasti, J. I., Gross, U., Pohl, S., Lugert, R., Weig, M., Schmidt-Ott, R. (2007). Role of the plasmid-encoded tet(O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli. J Med Microbiol 56: 833-837 [Abstract] [Full Text]  
  • Wardak, S., Szych, J., Zasada, A. A., Gierczynski, R. (2007). Antibiotic Resistance of Campylobacter jejuni and Campylobacter coli Clinical Isolates from Poland. Antimicrob. Agents Chemother. 51: 1123-1125 [Abstract] [Full Text]  
  • Oyarzabal, O. A., Rad, R., Backert, S. (2007). Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni. J. Clin. Microbiol. 45: 402-408 [Abstract] [Full Text]  
  • Inglis, G. D., Morck, D. W., McAllister, T. A., Entz, T., Olson, M. E., Yanke, L. J., Read, R. R. (2006). Temporal Prevalence of Antimicrobial Resistance in Campylobacter spp. from Beef Cattle in Alberta Feedlots.. Appl. Environ. Microbiol. 72: 4088-4095 [Abstract] [Full Text]  
  • Kos, V. N., Keelan, M., Taylor, D. E. (2006). Antimicrobial Susceptibilities of Campylobacter jejuni Isolates from Poultry from Alberta, Canada. Antimicrob. Agents Chemother. 50: 778-780 [Abstract] [Full Text]  
  • Thakur, S., Gebreyes, W. A. (2005). Campylobacter coli in Swine Production: Antimicrobial Resistance Mechanisms and Molecular Epidemiology. J. Clin. Microbiol. 43: 5705-5714 [Abstract] [Full Text]  
  • Fairchild, A. S., Smith, J. L., Idris, U., Lu, J., Sanchez, S., Purvis, L. B., Hofacre, C., Lee, M. D. (2005). Effects of Orally Administered Tetracycline on the Intestinal Community Structure of Chickens and on tet Determinant Carriage by Commensal Bacteria and Campylobacter jejuni. Appl. Environ. Microbiol. 71: 5865-5872 [Abstract] [Full Text]  
  • Gibreel, A., Kos, V. N., Keelan, M., Trieber, C. A., Levesque, S., Michaud, S., Taylor, D. E. (2005). Macrolide Resistance in Campylobacter jejuni and Campylobacter coli: Molecular Mechanism and Stability of the Resistance Phenotype. Antimicrob. Agents Chemother. 49: 2753-2759 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  
  • Pratt, A., Korolik, V. (2005). Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates. J Antimicrob Chemother 55: 452-460 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»