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Antimicrobial Agents and Chemotherapy, September 2004, p. 3586-3590, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3586-3590.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic and Culture-Based Approaches for Detecting Macrolide Resistance in Chlamydia pneumoniae

Paul F. Riska,1* Andrei Kutlin,2 Patrick Ajiboye,1 Arnold Cua,1 Patricia M. Roblin,2 and Margaret R. Hammerschlag1,2

Departments of Medicine,1 Pediatrics, State University of New York Downstate Medical Center, Brooklyn, New York2

Received 9 January 2004/ Returned for modification 8 March 2004/ Accepted 19 May 2004

Three clinical Chlamydia pneumoniae isolates for which the MIC of azithromycin increased after treatment were investigated for genetic evidence of macrolide resistance. Attempts to induce antibiotic resistance in vitro were made. No genetic mechanism was identified for the phenotypic change in these C. pneumoniae isolates. No macrolide resistance was obtained in vitro.

* Corresponding author. Mailing address: Box 56, 450 Clarkson Ave., Brooklyn, NY 11203. Phone: (718) 270-4181. Fax: (718) 270-4123. E-mail: riska{at}att.net.

Antimicrobial Agents and Chemotherapy, September 2004, p. 3586-3590, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3586-3590.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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