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Antimicrobial Agents and Chemotherapy, January 2005, p. 202-208, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.202-208.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

OXA-58, a Novel Class D ß-Lactamase Involved in Resistance to Carbapenems in Acinetobacter baumannii

Laurent Poirel,1 Sophie Marqué,1 Claire Héritier,1 Christine Segonds,2 Gérard Chabanon,2 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Université Paris XI, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre,1 Laboratoire de Bactériologie-Hygiène, CHU Rangeuil, Toulouse, France2

Received 1 April 2004/ Returned for modification 6 July 2004/ Accepted 11 September 2004

A carbapenem-resistant Acinetobacter baumannii strain was isolated in Toulouse, France, in 2003. Cloning and expression in Escherichia coli identified the carbapenem-hydrolyzing ß-lactamase OXA-58, which is weakly related (less than 50% amino acid identity) to other oxacillinases. It hydrolyzed penicillins, oxacillin, and imipenem but not expanded-spectrum cephalosporins. The blaOXA-58 gene was located on a ca. 30-kb non-self-transferable plasmid. After electrotransformation in the A. baumannii CIP7010T reference strain, it conferred reduced susceptibility to carbapenems. The blaOXA-58 gene was bracketed by two novel ISAba3-like insertion elements. This study describes a newly characterized ß-lactamase that may contribute to carbapenem resistance in A. baumannii.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, January 2005, p. 202-208, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.202-208.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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