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Antimicrobial Agents and Chemotherapy, January 2005, p. 202-208, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.202-208.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

OXA-58, a Novel Class D ß-Lactamase Involved in Resistance to Carbapenems in Acinetobacter baumannii

Service de Bactériologie-Virologie, Université Paris XI, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre,¹ Laboratoire de Bactériologie-Hygiène, CHU Rangeuil, Toulouse, France²

Received 1 April 2004/ Returned for modification 6 July 2004/ Accepted 11 September 2004

A carbapenem-resistant Acinetobacter baumannii strain was isolated in Toulouse, France, in 2003. Cloning and expression in Escherichia coli identified the carbapenem-hydrolyzing ß-lactamase OXA-58, which is weakly related (less than 50% amino acid identity) to other oxacillinases. It hydrolyzed penicillins, oxacillin, and imipenem but not expanded-spectrum cephalosporins. The bla_OXA-58 gene was located on a ca. 30-kb non-self-transferable plasmid. After electrotransformation in the A. baumannii CIP7010^T reference strain, it conferred reduced susceptibility to carbapenems. The bla_OXA-58 gene was bracketed by two novel ISAba3-like insertion elements. This study describes a newly characterized ß-lactamase that may contribute to carbapenem resistance in A. baumannii.

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