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Antimicrobial Agents and Chemotherapy, January 2005, p. 425-427, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.425-427.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

In-House Phage Amplification Assay Is a Sound Alternative for Detecting Rifampin-Resistant Mycobacterium tuberculosis in Low-Resource Settings

Instituto Nacional de Enfermedades Infecciosas, INEI-ANLIS "Carlos G. Malbrán," Buenos Aires, Argentina,¹ IVIC, Caracas, Venezuela,² London School of Hygiene and Tropical Medicine, London, United Kingdom,³ Institute of Tropical Medicine, Antwerp, Belgium⁴

Received 6 July 2004/ Returned for modification 20 August 2004/ Accepted 31 August 2004

An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.

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