This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Haanperä, M. Articles by Jalava, J. Search for Related Content PubMed PubMed Citation Articles by Haanperä, M. Articles by Jalava, J.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, January 2005, p. 457-460, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.457-460.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection and Quantification of Macrolide Resistance Mutations at Positions 2058 and 2059 of the 23S rRNA Gene by Pyrosequencing

Human Microbial Ecology Laboratory,¹ Antimicrobial Research Laboratory National Public Health Institute, Turku, Finland²

Received 24 June 2004/ Returned for modification 6 August 2004/ Accepted 7 September 2004

A pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (Escherichia coli numbering) of the 23S rRNA gene is described. The method was developed and tested for Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae.

This article has been cited by other articles:

• Haanpera, M., Forssten, S. D., Huovinen, P., Jalava, J. (2008). Typing of SHV Extended-Spectrum {beta}-Lactamases by Pyrosequencing in Klebsiella pneumoniae Strains with Chromosomal SHV {beta}-Lactamase. Antimicrob. Agents Chemother. 52: 2632-2635 [Abstract] [Full Text]  
• Moder, K.-A., Layer, F., Konig, W., Konig, B. (2007). Rapid screening of clarithromycin resistance in Helicobacter pylori by pyrosequencing. J Med Microbiol 56: 1370-1376 [Abstract] [Full Text]  
• Pandori, M. W., Gordones, C., Castro, L., Engelman, J., Siedner, M., Lukehart, S., Klausner, J. (2007). Detection of Azithromycin Resistance in Treponema pallidum by Real-Time PCR. Antimicrob. Agents Chemother. 51: 3425-3430 [Abstract] [Full Text]  
• Haanpera, M., Jalava, J., Huovinen, P., Meurman, O., Rantakokko-Jalava, K. (2007). Identification of Alpha-Hemolytic Streptococci by Pyrosequencing the 16S rRNA Gene and by Use of VITEK 2. J. Clin. Microbiol. 45: 762-770 [Abstract] [Full Text]  
• Naas, T., Oxacelay, C., Nordmann, P. (2007). Identification of CTX-M-Type Extended-Spectrum-{beta}-Lactamase Genes Using Real-Time PCR and Pyrosequencing. Antimicrob. Agents Chemother. 51: 223-230 [Abstract] [Full Text]  
• Poirel, L., Naas, T., Nordmann, P. (2006). Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum {beta}-Lactamases.. J. Clin. Microbiol. 44: 3008-3011 [Abstract] [Full Text]  
• Gibreel, A., Taylor, D. E. (2006). Macrolide resistance in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother 58: 243-255 [Abstract] [Full Text]  
• Jureen, P., Engstrand, L., Eriksson, S., Alderborn, A., Krabbe, M., Hoffner, S. E. (2006). Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis by Pyrosequencing Technology.. J. Clin. Microbiol. 44: 1925-1929 [Abstract] [Full Text]  
• Rantala, M., Haanpera-Heikkinen, M., Lindgren, M., Seppala, H., Huovinen, P., Jalava, J., the Finnish Study Group for Antimicrobial Resistan, (2006). Streptococcus pneumoniae Isolates Resistant to Telithromycin.. Antimicrob. Agents Chemother. 50: 1855-1858 [Abstract] [Full Text]  
• Gygax, S. E., Schuyler, J. A., Kimmel, L. E., Trama, J. P., Mordechai, E., Adelson, M. E. (2006). Erythromycin and clindamycin resistance in group B streptococcal clinical isolates.. Antimicrob. Agents Chemother. 50: 1875-1877 [Abstract] [Full Text]  
• Rantala, M., Huikko, S., Huovinen, P., Jalava, J., the Finnish Study Group for Antimicrobial Resistan, (2005). Prevalence and Molecular Genetics of Macrolide Resistance among Streptococcus pneumoniae Isolates Collected in Finland in 2002. Antimicrob. Agents Chemother. 49: 4180-4184 [Abstract] [Full Text]  
• Woodford, N., Sundsfjord, A. (2005). Molecular detection of antibiotic resistance: when and where?. J Antimicrob Chemother 56: 259-261 [Abstract] [Full Text]