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Antimicrobial Agents and Chemotherapy, January 2005, p. 470-472, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.470-472.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Tn1546-Like Elements in Vancomycin-Resistant Staphylococcus aureus Isolates from Michigan and Pennsylvania

Nancye C. Clark,* Linda M. Weigel, Jean B. Patel, and Fred C. Tenover

Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 2 July 2004/ Returned for modification 3 August 2004/ Accepted 23 September 2004

In 2002, the first two clinical isolates of vancomycin-resistant Staphylococcus aureus (VRSA) containing vanA were recovered in Michigan and Pennsylvania. Tn1546, a mobile genetic element that encodes high-level vancomycin resistance in enterococci, was present in both isolates. With PCR and DNA sequence analysis, we compared the Tn1546 elements from each isolate to the prototype Tn1546 element. The Michigan VRSA element was identical to the prototype Tn1546 element. The Pennsylvania VRSA element showed three distinct modifications: a deletion of nucleotides 1 to 3098 at the 5' end, which eliminated the orf1 region; an 809-bp IS1216V-like element inserted before nucleotide 3099 of Tn1546; and an inverted 1,499-bp IS1251-like element inserted into the vanSH intergenic region. These differences in the Tn1546-like elements indicate that the first two VRSA isolates were the result of independent genetic events.

* Corresponding author. Mailing address: Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-08 Atlanta, GA 30333. Phone: (404) 639-2625. Fax: (404) 639-1381. E-mail: ncc1{at}cdc.gov.

Antimicrobial Agents and Chemotherapy, January 2005, p. 470-472, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.470-472.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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