This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Clark, N. C. Articles by Tenover, F. C. Search for Related Content PubMed PubMed Citation Articles by Clark, N. C. Articles by Tenover, F. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, January 2005, p. 470-472, Vol. 49, No. 1
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.1.470-472.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Tn1546-Like Elements in Vancomycin-Resistant Staphylococcus aureus Isolates from Michigan and Pennsylvania

Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 2 July 2004/ Returned for modification 3 August 2004/ Accepted 23 September 2004

In 2002, the first two clinical isolates of vancomycin-resistant Staphylococcus aureus (VRSA) containing vanA were recovered in Michigan and Pennsylvania. Tn1546, a mobile genetic element that encodes high-level vancomycin resistance in enterococci, was present in both isolates. With PCR and DNA sequence analysis, we compared the Tn1546 elements from each isolate to the prototype Tn1546 element. The Michigan VRSA element was identical to the prototype Tn1546 element. The Pennsylvania VRSA element showed three distinct modifications: a deletion of nucleotides 1 to 3098 at the 5' end, which eliminated the orf1 region; an 809-bp IS1216V-like element inserted before nucleotide 3099 of Tn1546; and an inverted 1,499-bp IS1251-like element inserted into the vanSH intergenic region. These differences in the Tn1546-like elements indicate that the first two VRSA isolates were the result of independent genetic events.

This article has been cited by other articles:

• Zhu, W., Clark, N. C., McDougal, L. K., Hageman, J., McDonald, L. C., Patel, J. B. (2008). Vancomycin-Resistant Staphylococcus aureus Isolates Associated with Inc18-Like vanA Plasmids in Michigan. Antimicrob. Agents Chemother. 52: 452-457 [Abstract] [Full Text]  
• Saha, B., Singh, A. K., Ghosh, A., Bal, M. (2008). Identification and characterization of a vancomycin-resistant Staphylococcus aureus isolated from Kolkata (South Asia). J Med Microbiol 57: 72-79 [Abstract] [Full Text]  
• Fosheim, G. E., Carey, R. B., Limbago, B. M. (2007). Evaluation of the AdvanDx VRE EVIGENE Assay for Detection of vanA in Vancomycin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 1611-1613 [Abstract] [Full Text]  
• Perichon, B., Courvalin, P. (2006). Synergism between {beta}-Lactams and Glycopeptides against VanA-Type Methicillin-Resistant Staphylococcus aureus and Heterologous Expression of the vanA Operon. Antimicrob. Agents Chemother. 50: 3622-3630 [Abstract] [Full Text]  
• Gemmell, C. G., Edwards, D. I., Fraise, A. P., Gould, F. K., Ridgway, G. L., Warren, R. E., on behalf of the Joint Working Party of the Britis, (2006). Guidelines for the prophylaxis and treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections in the UK. J Antimicrob Chemother 57: 589-608 [Abstract] [Full Text]  
• Launay, A., Ballard, S. A., Johnson, P. D. R., Grayson, M. L., Lambert, T. (2006). Transfer of Vancomycin Resistance Transposon Tn1549 from Clostridium symbiosum to Enterococcus spp. in the Gut of Gnotobiotic Mice. Antimicrob. Agents Chemother. 50: 1054-1062 [Abstract] [Full Text]  
• Guardabassi, L., Perichon, B., van Heijenoort, J., Blanot, D., Courvalin, P. (2005). Glycopeptide Resistance vanA Operons in Paenibacillus Strains Isolated from Soil. Antimicrob. Agents Chemother. 49: 4227-4233 [Abstract] [Full Text]