This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodriguez-Tudela, J. L.
Right arrow Articles by Cuenca-Estrella, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodriguez-Tudela, J. L.
Right arrow Articles by Cuenca-Estrella, M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, October 2005, p. 4026-4034, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4026-4034.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Susceptibility Patterns and Molecular Identification of Trichosporon Species

Juan L. Rodriguez-Tudela,1* Teresa M. Diaz-Guerra,1 Emilia Mellado,1 Virginia Cano,2 Cecilia Tapia,3 Alexander Perkins,1 Alicia Gomez-Lopez,1 Laura Rodero,2 and Manuel Cuenca-Estrella1

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,1 Departamento Micología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán," Buenos Aires, Argentina,2 Programa de Microbiología y Micología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago de Chile, Chile3

Received 9 June 2005/ Returned for modification 5 July 2005/ Accepted 11 July 2005

The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 µg/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 µg/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC ≤0.14 µg/ml. The sequencing of IGS correctly identified Trichosporon isolates; however, this technique is not available in many clinical laboratories, and strains should be dispatched to reference centers where these complex methods are available. Therefore, it seems to be more practical to perform antifungal susceptibility testing of all isolates belonging to Trichosporon spp., since correct identification could take several weeks, delaying the indication of an antifungal agent which exhibits activity against the infectious strain.

* Corresponding author. Mailing address: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km. 2, 28220 Majadahonda (Madrid), Spain. Phone: 34-91-82236611. Fax: 34-91-5097966. E-mail: juanl.rodriguez-tudela{at}isciii.es.

Antimicrobial Agents and Chemotherapy, October 2005, p. 4026-4034, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4026-4034.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Taj-Aldeen, S. J., Al-Ansari, N., El Shafei, S., Meis, J. F., Curfs-Breuker, I., Theelen, B., Boekhout, T. (2009). Molecular Identification and Susceptibility of Trichosporon Species Isolated from Clinical Specimens in Qatar: Isolation of Trichosporon dohaense Taj-Aldeen, Meis & Boekhout sp. nov.. J. Clin. Microbiol. 47: 1791-1799 [Abstract] [Full Text]  
  • Chagas-Neto, T. C., Chaves, G. M., Melo, A. S. A., Colombo, A. L. (2009). Bloodstream Infections Due to Trichosporon spp.: Species Distribution, Trichosporon asahii Genotypes Determined on the Basis of Ribosomal DNA Intergenic Spacer 1 Sequencing, and Antifungal Susceptibility Testing. J. Clin. Microbiol. 47: 1074-1081 [Abstract] [Full Text]  
  • Lass-Florl, C., Alastruey-Izquierdo, A., Cuenca-Estrella, M., Perkhofer, S., Rodriguez-Tudela, J. L. (2009). In Vitro Activities of Various Antifungal Drugs against Aspergillus terreus: Global Assessment Using the Methodology of the European Committee on Antimicrobial Susceptibility Testing. Antimicrob. Agents Chemother. 53: 794-795 [Abstract] [Full Text]  
  • Pfaller, M. A., Diekema, D. J., Gibbs, D. L., Newell, V. A., Bijie, H., Dzierzanowska, D., Klimko, N. N., Letscher-Bru, V., Lisalova, M., Muehlethaler, K., Rennison, C., Zaidi, M., the Global Antifungal Surveillance Group, (2009). Results from the ARTEMIS DISK Global Antifungal Surveillance Study, 1997 to 2007: 10.5-Year Analysis of Susceptibilities of Noncandidal Yeast Species to Fluconazole and Voriconazole Determined by CLSI Standardized Disk Diffusion Testing. J. Clin. Microbiol. 47: 117-123 [Abstract] [Full Text]  
  • Castelli, M. V., Alastruey-Izquierdo, A., Cuesta, I., Monzon, A., Mellado, E., Rodriguez-Tudela, J. L., Cuenca-Estrella, M. (2008). Susceptibility Testing and Molecular Classification of Paecilomyces spp.. Antimicrob. Agents Chemother. 52: 2926-2928 [Abstract] [Full Text]  
  • Alastruey-Izquierdo, A., Cuenca-Estrella, M., Monzon, A., Mellado, E., Rodriguez-Tudela, J. L. (2008). Antifungal susceptibility profile of clinical Fusarium spp. isolates identified by molecular methods. J Antimicrob Chemother 61: 805-809 [Abstract] [Full Text]  
  • Alcazar-Fuoli, L., Mellado, E., Alastruey-Izquierdo, A., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2008). Aspergillus Section Fumigati: Antifungal Susceptibility Patterns and Sequence-Based Identification. Antimicrob. Agents Chemother. 52: 1244-1251 [Abstract] [Full Text]  
  • Alastruey-Izquierdo, A., Cuenca-Estrella, M., Monzon, A., Rodriguez-Tudela, J. L. (2007). Prevalence and Susceptibility Testing of New Species of Pseudallescheria and Scedosporium in a Collection of Clinical Mold Isolates. Antimicrob. Agents Chemother. 51: 748-751 [Abstract] [Full Text]  
  • Gunn, S. R., Reveles, X. T., Hamlington, J. D., Sadkowski, L. C., Johnson-Pais, T. L., Jorgensen, J. H. (2006). Use of DNA Sequencing Analysis To Confirm Fungemia Due to Trichosporon dermatis in a Pediatric Patient.. J. Clin. Microbiol. 44: 1175-1177 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»