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Antimicrobial Agents and Chemotherapy, October 2005, p. 4174-4179, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4174-4179.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of the Naturally Occurring Oxacillinase of Acinetobacter baumannii

Claire Héritier,1 Laurent Poirel,1 Pierre-Edouard Fournier,2,3 Jean-Michel Claverie,3 Didier Raoult,2 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 K.-Bicêtre,1 Unité des Rickettsies, IFR 48, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée,2 IGS, CNRS UPR2589, Marseille, France3

Received 24 February 2005/ Returned for modification 9 May 2005/ Accepted 24 July 2005

A chromosomally encoded oxacillinase, OXA-69, was characterized from Acinetobacter baumannii AYE. ß-Lactamase OXA-69 shared 97% amino acid identity with the recently described OXA-51 enzyme of A. baumannii and 62 and 56% amino acid identity with the carbapenem-hydrolyzing oxacillinases OXA-24 and OXA-23, respectively. Biochemical characterization of the purified OXA-69 revealed a narrow-spectrum hydrolysis profile but including, at a low level, imipenem and meropenem. By PCR and sequencing blaOXA-69-like genes were identified in all A. baumannii strains tested (n = 12), suggesting that this oxacillinase is naturally occurring in that species.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, October 2005, p. 4174-4179, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4174-4179.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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