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Antimicrobial Agents and Chemotherapy, October 2005, p. 4375-4378, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4375-4378.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction of the MexA and MexB Components of the MexAB-OprM Multidrug Efflux System of Pseudomonas aeruginosa: Identification of MexA Extragenic Suppressors of a T578I Mutation in MexB

Dominic Nehme and Keith Poole*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Received 2 May 2005/ Returned for modification 5 July 2005/ Accepted 17 July 2005

A T578I mutation in MexB compromised the protein's contribution to antimicrobial resistance and negatively impacted its interaction with MexA. Mutations causing single amino acid changes in the C-terminal domain of MexA (R221H, L245F, E254K, and V259I) suppressed the antimicrobial susceptibility of a MexBT578I-expressing Pseudomonas aeruginosa strain and restored a MexA interaction with MexBT578I. These data confirm the importance of the MexA C-terminal region in MexB binding and the likely significance of the region surrounding T587I of MexB in MexA interaction.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6. Phone: (613) 533-6677. Fax: (613) 533-6796. E-mail: poolek{at}post.queensu.ca.

Antimicrobial Agents and Chemotherapy, October 2005, p. 4375-4378, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4375-4378.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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