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Antimicrobial Agents and Chemotherapy, October 2005, p. 4400-4403, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4400-4403.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic and Enzymatic Properties of Metallo-ß-Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae

Gulcin G. Gacar,1 Kenan Midilli,2 Fetiye Kolayli,1 Kivanc Ergen,3 Sibel Gundes,4 Salih Hosoglu,5 Aynur Karadenizli,1 and Haluk Vahaboglu4*

Mikrobiyoloji and Klinik Mikrobiyoloji AD,1 Biofizik AD,3 Enfeksiyon Hastaliklari and Klinik Mikrobiyoloji AD, Kocaeli Universitesi, Kocaeli,4 Enfeksiyon Hastaliklari and Klinik Mikrobiyoloji AD, Dicle Universitesi, Diyarbakir,5 Mikrobiyoloji and Klinik Mikrobiyoloji AD, Cerrahpasa Tip Fakultesi, Istanbul Universitesi, Istanbul, Turkey2

Received 2 May 2005/ Returned for modification 11 July 2005/ Accepted 22 July 2005

A VIM-5-producing Enterobacter cloacae isolate (EDV/1) was identified in a collection of clinical strains stored before 2002. The gene, blaVIM-5, was located on a 2,712-bp BamHI-HindIII fragment of a 23-kbp (approximately) nonconjugative plasmid (pEDV5) in a class 1 integron as a single gene cassette.


* Corresponding author. Mailing address: KOU Tip Fakultesi Sopali ciftligi, Derince 41900 Kocaeli, Turkey. Phone: 90-262-3037560. Fax: 90-262-2335461. E-mail: vahabo{at}hotmail.com.


Antimicrobial Agents and Chemotherapy, October 2005, p. 4400-4403, Vol. 49, No. 10
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.10.4400-4403.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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