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Antimicrobial Agents and Chemotherapy, November 2005, p. 4530-4535, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4530-4535.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Antibiotic Treatment against "Candidatus Helicobacter suis" in a Mouse Model

A. Hellemans,* A. Decostere, F. Haesebrouck, and R. Ducatelle

Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

Received 8 June 2005/ Returned for modification 13 July 2005/ Accepted 19 August 2005

"Helicobacter heilmannii" (proposed name) type 1 colonizes the human stomach. It has been shown to be identical to "Candidatus Helicobacter suis," a Helicobacter species colonizing the stomachs of >60% of slaughter pigs. This bacterium has not been isolated in vitro until now. Antibiotic susceptibility testing of "Candidatus Helicobacter suis" has not been carried out so far. For the present study, a mouse model was adopted to evaluate the antibiotic susceptibility of this organism. Mice infected with "Candidatus Helicobacter suis" were treated with amoxicillin and omeprazole, a therapy which is used to treat H. heilmannii infections in humans. Two different isolates of "Candidatus Helicobacter suis" were tested. The excretion of bacterial DNA was assessed during treatment, using PCR on fecal samples. At the end of the experiment, 8 days after the cessation of treatment, the presence of infection was evaluated using a urease test and a PCR test on stomach samples. A marked decrease in the excretion of bacterial DNA was observed a few days after the onset of treatment, and the level remained low until the end of the experiment. A difference in susceptibility between the two "Candidatus Helicobacter suis" isolates was pointed out. The in vivo mouse model infected with "Candidatus Helicobacter suis" will be useful for further screening of potential therapeutic regimens.

* Corresponding author. Mailing address: Laboratory of Pathology, Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Phone: 32 9 264 77 45. Fax: 32 9 264 77 89. E-mail: ann.hellemans{at}Ugent.be.

Antimicrobial Agents and Chemotherapy, November 2005, p. 4530-4535, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4530-4535.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Haesebrouck, F., Pasmans, F., Flahou, B., Chiers, K., Baele, M., Meyns, T., Decostere, A., Ducatelle, R. (2009). Gastric Helicobacters in Domestic Animals and Nonhuman Primates and Their Significance for Human Health. Clin. Microbiol. Rev. 22: 202-223 [Abstract] [Full Text]  
  • Matsui, H., Aikawa, C., Sekiya, Y., Takahashi, S., Murayama, S. Y., Nakamura, M. (2008). Evaluation of Antibiotic Therapy for Eradication of "Candidatus Helicobacter heilmannii". Antimicrob. Agents Chemother. 52: 2988-2989 [Abstract] [Full Text]  
  • Baele, M., Decostere, A., Vandamme, P., Ceelen, L., Hellemans, A., Mast, J., Chiers, K., Ducatelle, R., Haesebrouck, F. (2008). Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs. Int. J. Syst. Evol. Microbiol. 58: 1350-1358 [Abstract] [Full Text]  
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