This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chmelnitsky, I.
Right arrow Articles by Navon-Venezia, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chmelnitsky, I.
Right arrow Articles by Navon-Venezia, S.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2005, p. 4745-4750, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4745-4750.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

CTX-M-2 and a New CTX-M-39 Enzyme Are the Major Extended-Spectrum Beta-Lactamases in Multiple Escherichia coli Clones Isolated in Tel Aviv, Israel

Inna Chmelnitsky, Yehuda Carmeli, Azita Leavitt, Mitchell J. Schwaber, and Shiri Navon-Venezia*

Division of Epidemiology and Laboratory for Molecular Epidemiology and Antibiotic Research, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

Received 14 April 2005/ Returned for modification 24 June 2005/ Accepted 3 August 2005

The rate of occurrence of the extended-spectrum beta-lactamase (ESBL)-producing phenotype among Escherichia coli isolates in Tel Aviv is 12% (22). The aim of this study was to understand the molecular epidemiology of E. coli ESBL producers and to identify the ESBL genes carried by them. We studied 20 single-patient ESBL-producing E. coli clinical isolates. They comprised 11 distinct nonrelated pulsed-field gel electrophoresis (PFGE) genotypes: six isolates belonged to the same PFGE clone, four other clones included two isolates each, and six unrelated clones included only one isolate. All isolates produced various beta-lactamases with pIs ranging from 5.2 to 8.2, varying within similar PFGE clones. The most prevalent ESBL gene was blaCTX-M; 16 isolates carried blaCTX-M-2 and three carried a new ESBL gene designated blaCTX-M-39. Three strains carried blaSHV (two blaSHV-12 and one blaSHV-5), and two strains carried inhibitor-resistant ESBL genes, blaTEM-33 and blaTEM-30; 18 strains carried blaTEM-1 and eight strains carried blaOXA-2. Plasmid mapping and Southern blot analysis with a CTX-M-2 probe demonstrated that blaCTX-M-2 is plasmid borne. The wide dissemination of ESBLs among E. coli isolates in our institution is partly related to clonal spread, but more notably to various plasmid-associated ESBL genes, occurring in multiple clones, wherein the CTX-M gene family appears almost uniformly. We report here a new CTX-M gene, designated blaCTX-M-39, which revealed 99% homology with blaCTX-M-26, with a substitution of arginine for glutamine at position 225.

* Corresponding author. Mailing address: Division of Epidemiology, Tel Aviv Sourasky Medical Center, 6 Weizmann St., Tel Aviv 64239, Israel. Phone: 972 3 692 5644. Fax: 972 3 697 4966. E-mail: shiri_nv{at}tasmc.health.gov.il.

Antimicrobial Agents and Chemotherapy, November 2005, p. 4745-4750, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4745-4750.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Leavitt, A., Chmelnitsky, I., Colodner, R., Ofek, I., Carmeli, Y., Navon-Venezia, S. (2009). Ertapenem Resistance among Extended-Spectrum-{beta}-Lactamase-Producing Klebsiella pneumoniae Isolates. J. Clin. Microbiol. 47: 969-974 [Abstract] [Full Text]  
  • Wolter, D. J., Kurpiel, P. M., Woodford, N., Palepou, M.-F. I., Goering, R. V., Hanson, N. D. (2009). Phenotypic and Enzymatic Comparative Analysis of the Novel KPC Variant KPC-5 and Its Evolutionary Variants, KPC-2 and KPC-4. Antimicrob. Agents Chemother. 53: 557-562 [Abstract] [Full Text]  
  • Wu, Q., Zhang, Y., Han, L., Sun, J., Ni, Y. (2009). Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China. Antimicrob. Agents Chemother. 53: 271-272 [Abstract] [Full Text]  
  • Woodford, N., Zhang, J., Warner, M., Kaufmann, M. E., Matos, J., MacDonald, A., Brudney, D., Sompolinsky, D., Navon-Venezia, S., Livermore, D. M. (2008). Arrival of Klebsiella pneumoniae producing KPC carbapenemase in the United Kingdom. J Antimicrob Chemother 62: 1261-1264 [Abstract] [Full Text]  
  • Navon-Venezia, S., Chmelnitsky, I., Leavitt, A., Carmeli, Y. (2008). Dissemination of the CTX-M-25 family {beta}-lactamases among Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae and identification of the novel enzyme CTX-M-41 in Proteus mirabilis in Israel. J Antimicrob Chemother 62: 289-295 [Abstract] [Full Text]  
  • Bhattacharjee, A., Sen, M. R., Prakash, P., Anupurba, S. (2008). Role of {beta}-lactamase inhibitors in enterobacterial isolates producing extended-spectrum {beta}-lactamases. J Antimicrob Chemother 61: 309-314 [Abstract] [Full Text]  
  • Bhattacharjee, A., Sen, M. R., Anupurba, S., Prakash, P., Nath, G. (2007). Detection of OXA-2 group extended-spectrum-{beta}-lactamase-producing clinical isolates of Escherichia coli from India. J Antimicrob Chemother 60: 703-704 [Full Text]  
  • Fernandez, A., Gil, E., Cartelle, M., Perez, A., Beceiro, A., Mallo, S., Tomas, M. M., Perez-Llarena, F. J., Villanueva, R., Bou, G. (2007). Interspecies spread of CTX-M-32 extended-spectrum {beta}-lactamase and the role of the insertion sequence IS1 in down-regulating blaCTX-M gene expression. J Antimicrob Chemother 59: 841-847 [Abstract] [Full Text]  
  • Ensor, V. M., Livermore, D. M., Hawkey, P. M. (2007). A novel reverse-line hybridization assay for identifying genotypes of CTX-M-type extended-spectrum {beta}-lactamases. J Antimicrob Chemother 59: 387-395 [Abstract] [Full Text]  
  • Livermore, D. M., Canton, R., Gniadkowski, M., Nordmann, P., Rossolini, G. M., Arlet, G., Ayala, J., Coque, T. M., Kern-Zdanowicz, I., Luzzaro, F., Poirel, L., Woodford, N. (2007). CTX-M: changing the face of ESBLs in Europe. J Antimicrob Chemother 59: 165-174 [Abstract] [Full Text]  
  • Navon-Venezia, S., Chmelnitsky, I., Leavitt, A., Schwaber, M. J., Schwartz, D., Carmeli, Y. (2006). Plasmid-Mediated Imipenem-Hydrolyzing Enzyme KPC-2 among Multiple Carbapenem-Resistant Escherichia coli Clones in Israel.. Antimicrob. Agents Chemother. 50: 3098-3101 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»