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Antimicrobial Agents and Chemotherapy, November 2005, p. 4771-4774, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4771-4774.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Recombination between the dfrA12-orfF-aadA2 Cassette Array and an aadA1 Gene Cassette Creates a Hybrid Cassette, aadA8b

Alicia M. Gestal,1 H. W. Stokes,1 Sally R. Partridge,1,{dagger} and Ruth M. Hall2*

Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109,1 School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia2

Received 20 December 2004/ Returned for modification 22 February 2005/ Accepted 19 August 2005

Homologous recombination between closely related gene cassettes, such as aadA1 and aadA2, which are 89% identical, can create hybrid cassettes and hybrids of existing cassette arrays. A new cassette array, dfrA12-orfF-aadA8b, which was created by such a recombination event occurring within the aadA2 cassette in the dfrA12-orfF-aadA2 array, has been identified.

* Corresponding author. Mailing address: School of Molecular and Microbial Biosciences, Biochemistry and Microbiology Building G08, The University of Sydney, NSW 2006, Australia. Phone: 61-2-9351-6014. Fax: 61-2-9351-4571. E-mail: Ruth.Hall{at}mmb.usyd.edu.au.

{dagger} Present address: Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, NSW 2145, Australia

Antimicrobial Agents and Chemotherapy, November 2005, p. 4771-4774, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4771-4774.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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