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Antimicrobial Agents and Chemotherapy, November 2005, p. 4775-4777, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4775-4777.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

mmpL7 Gene of Mycobacterium tuberculosis Is Responsible for Isoniazid Efflux in Mycobacterium smegmatis

Maria R. Pasca,1 Paola Guglierame,1 Edda De Rossi,1 Francesca Zara,2 and Giovanna Riccardi1*

Dipartimento di Genetica e Microbiologia,1 Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, University of Pavia, Pavia, Italy2

Received 23 June 2005/ Returned for modification 21 July 2005/ Accepted 12 August 2005

The Mycobacterium tuberculosis mmpL7 gene, encoding a hypothetical resistance nodulation division transporter, confers a high resistance level to isoniazid when overexpressed in Mycobacterium smegmatis. The resistance level decreased in the presence of the efflux pump inhibitors reserpine and CCCP (carbonyl cyanide m-chlorophenylhydrazone). Energy-dependent efflux of isoniazid from M. smegmatis cells expressing the mmpL7 gene was observed.

* Corresponding author. Mailing address: Dipartimento di Genetica e Microbiologia, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy. Phone: 39 0382 985574. Fax: 39 0382 528496. E-mail: riccardi{at}ipvgen.unipv.it

Antimicrobial Agents and Chemotherapy, November 2005, p. 4775-4777, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4775-4777.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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