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Antimicrobial Agents and Chemotherapy, November 2005, p. 4798-4800, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4798-4800.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Simultaneous Detection of Macrolide and Tetracycline Resistance Determinants in Streptococci

Surbhi Malhotra-Kumar,* Christine Lammens, Jasper Piessens, and Herman Goossens

Belgian Reference Center for Group A Streptococcus, University of Antwerp, Antwerp, Belgium

Received 31 May 2005/ Returned for modification 29 July 2005/ Accepted 26 August 2005

Resistance to macrolides and tetracyclines is increasing among streptococci and co-occurs as their resistance determinants are carried on the same mobile element. We developed a multiplex PCR to facilitate simultaneous and specific detection of resistance determinants for both macrolides [erm(A), erm(B), and mef(A/E)] and tetracyclines [tet(M), tet(O), tet(K), and tet(L)] in streptococci.

* Corresponding author. Mailing address: Department of Medical Microbiology, Campus Drie Eiken, University of Antwerp, S3, Universiteitsplein 1, B-2610 Wilrijk, Belgium. Phone: 32-3-820-25-51. Fax: 32-3-820-26-63. E-mail: surbhi.malhotra{at}ua.ac.be.

Antimicrobial Agents and Chemotherapy, November 2005, p. 4798-4800, Vol. 49, No. 11
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.11.4798-4800.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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