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Antimicrobial Agents and Chemotherapy, February 2005, p. 690-698, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.690-698.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Prevalence and Subtypes of Ciprofloxacin-Resistant Campylobacter spp. in Commercial Poultry Flocks before, during, and after Treatment with Fluoroquinolones

Tom J. Humphrey,1 Frieda Jørgensen,2 Jennifer A. Frost,3 Haddy Wadda,3 Gil Domingue,4 Nicola C. Elviss,2 Deborah J. Griggs,5 and Laura J. V. Piddock5*

School of Clinical Veterinary Science,1 Food Microbiology Collaborating Unit, Health Protection Agency South West, School of Clinical Veterinary Science, University of Bristol, Langford, Bristol,2 Campylobacter Reference Unit, Health Protection Agency Specialist & Reference Microbiology Division, Colindale, London,3 Avigen Ltd., Broxburn, West Lothian, Scotland,4 Antimicrobial Agents Research Group, Division of Immunity & Infection, University of Birmingham Medical School, Birmingham, United Kingdom5

Received 20 January 2004/ Returned for modification 11 June 2004/ Accepted 26 September 2004

Five commercial broiler chicken flocks were treated with either difloxacin or enrofloxacin for a clinically relevant infection, as instructed by a veterinarian. Campylobacters were isolated from individual fecal samples and from samples associated with the broiler environment before, during, and after treatment. Ciprofloxacin-resistant Campylobacter jejuni and/or C. coli strains were detected pretreatment in four flocks, but they constituted a very small proportion of the campylobacters present. When the broilers were treated with a fluoroquinolone, a rapid increase in the proportion of ciprofloxacin-resistant campylobacters was observed. During treatment nearly 100% of campylobacters were resistant, and in some flocks a high proportion of resistant strains persisted for up to 4 weeks after treatment. Prior to treatment a variety of campylobacter subtypes were present. During and after treatment considerable changes in both species and subtype prevalence were observed, but no single fluoroquinolone-resistant clone became dominant. Instead, resistant C. coli strains or a mixture of resistant C. coli and C. jejuni strains became dominant, whereas susceptible C. jejuni strains had usually been dominant prior to treatment. The resistant subtypes which emerged and became dominant were not always the same as those detected pretreatment. The persistence of resistant strains for up to 4 weeks posttreatment has important implications for any strategy designed to avoid the introduction of such strains into the food chain.

* Corresponding author. Mailing address: Antimicrobial Agents Research Group, Division of Immunity & Infection, University of Birmingham Medical School, Birmingham B15 2TT, United Kingdom. Phone: 0121 414 6966. Fax: 0121 414 3454. E-mail: l.j.v.piddock{at}bham.ac.uk.

Antimicrobial Agents and Chemotherapy, February 2005, p. 690-698, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.690-698.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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