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Antimicrobial Agents and Chemotherapy, February 2005, p. 801-803, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.801-803.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cloning of a Novel Gene for Quinolone Resistance from a Transferable Plasmid in Shigella flexneri 2b

Mami Hata,1* Masahiro Suzuki,1 Masakado Matsumoto,1 Masao Takahashi,1 Katsuhiko Sato,1 Shiro Ibe,1,2 and Kenji Sakae1

Department of Microbiology, Aichi Prefectural Institute of Public Health,1 Clinical Research Center, Nagoya Medical Center, Nagoya, Aichi, Japan2

Received 30 August 2004/ Returned for modification 26 September 2004/ Accepted 13 October 2004

A novel gene for quinolone resistance was cloned from a transferable plasmid carried by a clinical isolate of Shigella flexneri 2b that was resistant to fluoroquinolones. The plasmid conferred low-level resistance to quinolones on Escherichia coli HB101. The protein encoded by the gene showed 59% amino acid identity with Qnr.

* Corresponding author. Mailing address: Department of Microbiology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsujimachi, Kita-ku, Nagoya, Aichi 462-8576, Japan. Phone: 81-52-910-5674. Fax: 81-52-913-3641. E-mail: mami_hata{at}pref.aichi.lg.jp.

Antimicrobial Agents and Chemotherapy, February 2005, p. 801-803, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.801-803.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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