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Antimicrobial Agents and Chemotherapy, February 2005, p. 804-807, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.804-807.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Simple Fibroblast-Based Assay To Test the Pyrazinamide Susceptibility of Mycobacterium tuberculosis

Takemasa Takii,1* Sonomi Hamasaki,2 Kazue Hirano,2 Chiyoji Abe,2 and Kikuo Onozaki1

Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya,1 Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose, Tokyo, Japan2

Received 4 May 2004/ Returned for modification 5 August 2004/ Accepted 11 October 2004

A simple fibroblast-based assay (SFA) was found to be efficient in evaluating the susceptibilities of clinical isolates of Mycobacterium tuberculosis to pyrazinamide (PZA). Forty-five clinical isolates were examined. The MICs of PZA for susceptible strains in an SFA were between 3.13 and 12.5 µg/ml, and the MICs of PZA for resistant strains were more than 100 µg/ml.

* Corresponding author. Mailing address: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-Dori, Mizuho-Ku, Nagoya 467-8603, Japan. Phone: 81-52-836-3421. Fax: 81-52-836-3419. E-mail: ttakii{at}phar.nagoya-cu.ac.jp.

Antimicrobial Agents and Chemotherapy, February 2005, p. 804-807, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.804-807.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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