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Antimicrobial Agents and Chemotherapy, February 2005, p. 849-852, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.849-852.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Selected Arylpiperazines Are Capable of Reversing Multidrug Resistance in Escherichia coli Overexpressing RND Efflux Pumps

Jürgen A. Bohnert and Winfried V. Kern*

Center for Infectious Diseases and Travel Medicine, Department of Medicine, University Hospital, Freiburg, Germany

Received 11 May 2004/ Returned for modification 23 July 2004/ Accepted 24 October 2004

Several arylpiperazines capable of reversing multidrug resistance (MDR) in Escherichia coli overexpressing acrAB and acrEF but not in pump-deficient mutant strains were identified. 1-(1-Naphthylmethyl)-piperazine, one of the more active compounds, enhanced susceptibility to fluoroquinolones and other agents and increased the intracellular concentration of levofloxacin and ethidium bromide, suggesting efflux pump inhibition as the mechanism of MDR reversal.

* Corresponding author. Mailing address: Medizinische Universitätsklinik und Poliklinik, D-79106 Freiburg, Germany. Phone: 49-761-270 1819. Fax: 49-761-270 1820. E-mail: info{at}if-freiburg.de.

Antimicrobial Agents and Chemotherapy, February 2005, p. 849-852, Vol. 49, No. 2
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.2.849-852.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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