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Antimicrobial Agents and Chemotherapy, April 2005, p. 1364-1368, Vol. 49, No. 4
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.4.1364-1368.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method

Wendy W. J. van de Sande,1* Ad Luijendijk,1 Abdalla O. A. Ahmed,2 Irma A. J. M. Bakker-Woudenberg,1 and Alex van Belkum1

Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands,1 Mycetoma Research Group, Institute of Endemic Diseases and Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan2

Received 27 May 2004/ Returned for modification 11 July 2004/ Accepted 16 December 2004

The in vitro susceptibilities of 36 clinical isolates of Madurella mycetomatis, the prime agent of eumycetoma in Africa, to ketoconazole, itraconazole, fluconazole, voriconazole, amphotericin B, and flucytosine were determined by the Sensititre YeastOne system. This system appeared to be a rapid and easy test, and by use of hyphal suspensions it generated results comparable to those of a modified NCCLS method. After 10 days of incubation, the antifungal activities of ketoconazole (MIC at which 90% of isolates were inhibited [MIC90], 0.125 µg/ml), itraconazole (MIC90, 0.064 µg/ml), and voriconazole (MIC90, 0.125 µg/ml) appeared superior to those of fluconazole (MIC90, 128 µg/ml) and amphotericin B (MIC90, 1 µg/ml), with MICs in the clinically relevant range. All isolates were resistant to flucytosine (all MICs above 64 µg/ml). Based on the relatively broad range of MICs obtained for the antifungal agents, routine testing of M. mycetomatis isolates for susceptibility to antifungal agents seems to be relevant to adequate therapeutic management.

* Corresponding author. Mailing address: Erasmus MC, University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31-10-4635813. Fax: 31-10-4633875. E-mail: w.vandesande{at}erasmusmc.nl.

Antimicrobial Agents and Chemotherapy, April 2005, p. 1364-1368, Vol. 49, No. 4
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.4.1364-1368.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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