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Antimicrobial Agents and Chemotherapy, April 2005, p. 1564-1566, Vol. 49, No. 4
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.4.1564-1566.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Novel TEM-Type Extended-Spectrum ß-Lactamase, TEM-134, in a Citrobacter koseri Clinical Isolate

Mariagrazia Perilli,1 Claudia Mugnaioli,2 Francesco Luzzaro,3 Marianna Fiore,1 Stefania Stefani,4 Gian Maria Rossolini,2 and Gianfranco Amicosante1*

Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, L'Aquila,1 Dipartimento di Biologia Molecolare, Università di Siena, Siena,2 Ospedale di Circolo di Varese, Varese,3 Dipartimento di Scienze Microbiologiche e Ginecologiche, Sez. di Microbiologia, Università di Catania, Catania, Italy4

Received 19 July 2004/ Returned for modification 16 September 2004/ Accepted 28 November 2004

A new natural TEM derivative with extended-spectrum ß-lactamase activity, TEM-134, was identified in a ceftazidime-resistant clinical isolate of Citrobacter koseri. Compared to TEM-1, TEM-134 contains the following mutations: Q39K, E104K, R164H, and G238S. The blaTEM-134 gene was not transferable by conjugation and, apparently, was chromosomally encoded. Expression studies with Escherichia coli revealed efficient cefotaximase and ceftazidimase activity for TEM-134.

* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, Cattedra di Biochimica Clinica e Biologia Molecolare Clinica, Loc. Coppito, I-67100 L'Aquila, Italy. Phone: 39 0862 433455. Fax: 39 0862 433433. E-mail: amicosante{at}cc.univaq.it.

Antimicrobial Agents and Chemotherapy, April 2005, p. 1564-1566, Vol. 49, No. 4
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.4.1564-1566.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Perilli, M., Celenza, G., Fiore, M., Segatore, B., Pellegrini, C., Luzzaro, F., Rossolini, G. M., Amicosante, G. (2007). Biochemical analysis of TEM-134, a new TEM-type extended-spectrum {beta}-lactamase variant produced in a Citrobacter koseri clinical isolate from an Italian hospital. J Antimicrob Chemother 60: 877-880 [Abstract] [Full Text]  


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