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Antimicrobial Agents and Chemotherapy, May 2005, p. 1782-1786, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.1782-1786.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutations in PA3574 (nalD) Lead to Increased MexAB-OprM Expression and Multidrug Resistance in Laboratory and Clinical Isolates of Pseudomonas aeruginosa

Mara L. Sobel,1 Didier Hocquet,2 Lily Cao,1 Patrick Plesiat,2 and Keith Poole1*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6,1 Laboratoire de Bacteriologie, Hopital Jean Minjoz, F-25030 Besançon, France2

Received 6 December 2004/ Returned for modification 12 January 2005/ Accepted 19 January 2005

Mutations in genes mexR and nalC have previously been shown to drive overexpression of the MexAB-OprM multidrug efflux system in Pseudomonas aeruginosa. A transposon insertion multidrug-resistant mutant of P. aeruginosa overproducing MexAB-OprM was disrupted in yet a third gene, PA3574, encoding a probable repressor of the TetR/AcrR family that we have dubbed NalD. Clinical strains overexpressing MexAB-OprM but lacking mutations in mexR or nalC were also shown to carry mutations in nalD. Moreover, the cloned nalD gene reduced the multidrug resistance and MexAB-OprM expression of the transposon mutant and clinical isolates, highlighting the significance of the nalD mutations vis-à-vis MexAB-OprM overexpression in these isolates.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Rm. 737, Botterell Hall, Queen's University, Kingston, Ontario K7L 3N6, Canada. Phone: (613) 533-6677. Fax: (613) 533-6796. E-mail: poolek{at}post.queensu.ca.

Antimicrobial Agents and Chemotherapy, May 2005, p. 1782-1786, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.1782-1786.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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