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Antimicrobial Agents and Chemotherapy, May 2005, p. 1823-1829, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.1823-1829.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Integron Content of Extended-Spectrum-ß-Lactamase-Producing Escherichia coli Strains over 12 Years in a Single Hospital in Madrid, Spain

Hospital Universitario Ramón y Cajal, IMSALUD, Madrid, Spain,¹ Faculdade de Farmácia, Universidade do Porto, Porto, Portugal²

Received 8 August 2004/ Returned for modification 24 October 2004/ Accepted 23 January 2005

The contribution of integrons to the dissemination of extended-spectrum ß-lactamases (ESBL) was analyzed on all ESBL-producing Escherichia coli isolates from 1988 to 2000 at Ramón y Cajal Hospital. We studied 133 E. coli pulsed-field gel electrophoresis types: (i) 52 ESBL-producing clinical strains (C-ESBL) (16 TEM, 9 SHV, 21 CTX-M-9, 1 CTX-M-14, and 5 CTX-M-10); (ii) 43 non-ESBL blood clinical strains (C-nESBL); and (iii) 38 non-ESBL fecal isolates from healthy volunteers (V-nESBL). Class 1 integrons were more common among C-ESBL (67%) than among C-nESBL (40%) or V-nESBL (26%) (P < 0.001) due to the high number of strains with bla_CTX-M-9, which is linked to an In6-like class 1 integron. Without this bias, class 1 integron occurrence would be similar in C-ESBL and C-nESBL groups (47% versus 40%). Occurrence of class 2 integrons was similar among clinical and community isolates (13 to 18%). No isolates contained class 3 integrons. The relatively low rate of class 1 integrons within transferable elements carrying bla_TEM (23%) or bla_SHV (33%) and the absence of class 2 integrons in all ESBL transconjugants mirror the assembly of translocative pieces containing bla_TEM or bla_SHV on local available transferable elements lacking integrons. The low diversity of class 1 integrons (seven types recovered in all groups) might indicate a wide dissemination of specific genetic elements in which they are located. In our environment, the spread of genetic elements encoding ESBL has no major impact on the dispersion of integrons, nor do integrons have a major impact on the spread of ESBL, except when bla_ESBL genes are within an integron platform such as bla_CTX-M-9.

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