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Antimicrobial Agents and Chemotherapy, May 2005, p. 1927-1931, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.1927-1931.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Plasmid-Encoded Tet B Tetracycline Resistance in Haemophilus parasuis

John F. Lancashire,1 Tamsin D. Terry,1 P. J. Blackall,2 and Michael P. Jennings1*

School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia, Queensland 4072, Australia,1 Department of Primary Industries and Fisheries, Animal Research Institute, Yeerongpilly, Australia2

Received 9 June 2004/ Returned for modification 17 August 2004/ Accepted 19 January 2005

The complete sequence of two plasmids, pHS-Tet (5.1 kb) and pHS-Rec (9.5 kb), isolated from Haemophilus parasuis strain HS1543 has been obtained. Plasmid pHS-Tet contains four open reading frames including a tet(B) tetracycline resistance gene which unusually did not have an associated tetR repressor gene. From a total of 45 H. parasuis isolates surveyed (15 international reference strains, 15 field isolates selected for their genetic diversity, and 15 recent Australian field isolates), 2 tetracycline-resistant field isolates (HS226 and HS1859) were identified. Analysis of three additional isolates from the same disease outbreak as strain HS1859 revealed a further tetracycline-resistant H. parasuis strain (HS1857, serovar 8) and a tetracycline-resistant Actinobacillus pleuropneumoniae strain (HS1861). An approximately 10.6-kb plasmid was identified in field isolate HS226 and outbreak strains HS1857, HS1859, and HS1861. Southern hybridization analysis of these plasmids showed that the Tet B determinant was present, and restriction digest comparisons suggest that these plasmids are related. This is believed to be the first report of native H. parasuis plasmids and Tet B-mediated tetracycline resistance in this microorganism.

* Corresponding author: Mailing address: School of Molecular and Microbial Sciences, University of Queensland, Brisbane 4072, Australia. Phone: 61 7 3365 4879. Fax: 61 7 3365 4620. E-mail: jennings{at}uq.edu.au.

Antimicrobial Agents and Chemotherapy, May 2005, p. 1927-1931, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.1927-1931.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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