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Antimicrobial Agents and Chemotherapy, May 2005, p. 2122-2125, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.2122-2125.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nationwide Study of Escherichia coli and Klebsiella pneumoniae Producing Extended-Spectrum ß-Lactamases in Spain

J. R. Hernández,^1* L. Martínez-Martínez,^1,2, R. Cantón,³ T. M. Coque,³ A. Pascual,^1,2 Spanish Group for Nosocomial Infections (GEIH),

Department of Microbiology, University Hospital Virgen Macarena,¹ Department of Microbiology, University of Seville, Seville,² Department of Microbiology, University Hospital Ramon y Cajal, Madrid, Spain³

Received 8 March 2004/ Returned for modification 31 May 2004/ Accepted 7 January 2005

Clonal dissemination of extended-spectrum ß-lactamases (ESBL) in 170 Escherichia coli isolates and 70 Klebsiella pneumoniae isolates from a nationwide study of 40 Spanish centers in 2000 was not observed in most centers. The most prevalent ESBL were CTX-M-9 (27.3%), SHV-12 (23.9%), and CTX-M-14 (20.5%) for E. coli and TEM-3 (16.7%) and TEM-4 (25%) for K. pneumoniae. A new ESBL, TEM-133, with mutations L21F, E104K, and R164S, was identified.

Present address: Department of Microbiology, University Hospital Marqués de Valdecilla, Santander, Spain.

Study group GEIH-BLEE 2000 is represented by L. Michaus (Vitoria, Álava), C. Martínez Peinado (Villajoyosa, Alicante), A. Yagüe (Orihuela, Alicante), A. Torreblanca (Cangas del Narcea, Asturias), A. Fleites (Oviedo, Asturias), J. J. Moreno (Mérida, Badajoz), J. Gil (Palma de Mallorca, Baleares), M. A. Domínguez (Barcelona), F. Marco (Barcelona), O. del Valle (Barcelona), F. Corcoy (San Pedro de Rivas, Barcelona), E. Ojeda (Burgos), P. Marín (Cádiz), C. Fernández (Santander, Cantabria), R. Carranza (Alcázar de S. Juan, Ciudad Real), F. Rodríguez (Córdoba), I. Álamo (Gran Canaria), M. De la Rosa (Granada), J. Gutiérrez (Granada), M. Gomáriz (San Sebastián, Guipúzcoa), I. Cuesta (Jaén), M. Cartelle (La Coruña), M. Rodríguez (Ferrol, La Coruña), R. Cantón (Madrid), E. Cercenado (Madrid), L. Folgueira (Madrid), A. Delgado Iribarren (Alcorcón, Madrid), L. Torroba (Pamplona, Navarra), J. J. García Irure (Pamplona, Navarra), B. Fernández (Orense), F. Lueiro (Pontevedra), I. Otero (Vigo, Pontevedra), E. García Sánchez (Salamanca), J. R. Hernández (Seville), A. Moreno (Tenerife), C. Aspiroz (Teruel), E. García Leoni (Toledo), C. Ezpeleta (Bilbao, Vizcaya), J. Castillo (Zaragoza), and J. García Moya (Zaragoza).

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