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Antimicrobial Agents and Chemotherapy, May 2005, p. 2126-2129, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.2126-2129.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

High Genetic Diversity of Ciprofloxacin-Nonsusceptible Isolates of Streptococcus pneumoniae in Poland

Ewa Sadowy,* Radoslaw Izdebski, Anna Skoczynska, Marek Gniadkowski, and Waleria Hryniewicz

National Institute of Public Health, Chelmska 30/34, 00-725 Warsaw, Poland

Received 18 November 2004/ Returned for modification 24 December 2004/ Accepted 27 January 2005

We have analyzed the susceptibility to ciprofloxacin of 697 pneumococcal isolates collected in 1998-2002 in Poland from patients with respiratory tract diseases. Thirty-one ciprofloxacin-nonsusceptible isolates (MICs, ≥4 µg/ml) were identified, of which two were resistant to levofloxacin (MIC, 8 µg/ml). Serotyping, pulsed-field gel electrophoresis, multilocus sequence typing, and the analysis of resistance determinants showed their great genetic diversity.


* Corresponding author. Mailing address: Department of Molecular Microbiology, National Institute of Public Health, ul. Chelmska 30/34, 00-725 Warsaw, Poland. Phone: 48 22 851 43 88. Fax: 48 22 841 29 49. E-mail: ewasadowy{at}cls.edu.pl.


Antimicrobial Agents and Chemotherapy, May 2005, p. 2126-2129, Vol. 49, No. 5
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.5.2126-2129.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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